(provided by application): Active surveillance of acute gastroenteritis (AGE) will be conducted in the Emergency Dept. and in hospitalized patients who are seen at Children's Hospital &Research Center, Oakland (Oakland, CA), with the aims of determining the burden of AGE, the burden of rotavirus AGE, and the burden of norovirus AGE in children 15 days to <10 years of age. Clinical, demographic, and vaccination information will be collected for each patient. A stool sample will be collected from each patient within 14 days of onset of AGE symptoms. Stool samples will be tested on site by enzyme immunoassay (EIA), to detect rotaviruses. Stool samples will be tested for noroviruses by real-time polymerase chain reaction (RT-PCR). All stool samples demonstrating a positive result will be forwarded to CDC, for serotyping. Saliva samples will be obtained in a portion of AGE cases for determination of norovirus-secretor status. Age-matched children with acute respiratory infection (ARI) will be assigned for rotavirus cases, and age-matched normal children will be assigned for norovirus AGE cases. Population-based incidence rates by age will be determined for each of the following: total AGE, RV AGE and norovirus AGE, in hospitalized patients as well as patient seen only in the ED.

Public Health Relevance

Surveillance of hospitalized children will be conducted 24 hours/day, 7 days/week, 12 months/year. ED surveillance will be carried out 5 days/week for 6-8 hour shifts per day, 12 months/year. Stool and saliva samples are collected, as well as clinical, demographic, and vaccination records for each patient.

Agency
National Institute of Health (NIH)
Type
Research Project--Cooperative Agreements (U01)
Project #
3U01IP000463-03S1
Application #
8810759
Study Section
Special Emphasis Panel (ZIP1)
Program Officer
Loveys, Deborah
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Children's Hospital & Res Ctr at Oakland
Department
Type
DUNS #
City
Oakland
State
CA
Country
United States
Zip Code
94609