The goal of this application is to develop SYNPLA, a specific, selective and high-throughput method for marking experience-induced plasticity with single synapse resolution. SYNPLA exploits our understanding of the molecular mechanism of long-term potentiation, which is mediated by the insertion of a specific subtype of glutamate receptor at recently potentiated synapses.
In Aim 1 we develop SYNPLA in cultured neurons.
In Aims 2 and 3 we apply it to two brain circuits, cortico-amygdalar and cortico-striatal respectively which are known to undergo experience dependent plasticity. SYNPLA has the potential to emerge as a powerful tool for dissecting the circuit mechanisms underlying behavioral plasticity.

Public Health Relevance

This application will develop SYNPLA, a specific, selective and high-throughput method for marking experience-induced plasticity with single synapse resolution. SYNPLA has the potential to emerge as a powerful tool for dissecting the circuit mechanisms underlying behavioral plasticity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01MH109113-03
Application #
9301350
Study Section
Special Emphasis Panel (ZMH1)
Program Officer
Freund, Michelle
Project Start
2015-09-25
Project End
2018-06-30
Budget Start
2017-07-01
Budget End
2018-06-30
Support Year
3
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724