Sample Core will be in charge of the collection, isolation of specific cells, and dispatching cell samples. The type of samples will contain 1) blood draw, 2) blood apheresis, and 3) nasal wash samples. In particular, all the samples obtained at the Clinical Core will be transferred to the Sample Core, and stored after cell isolation, if required. The Core also will perform cell phenotyping by polychromatic flow cytometry and the gene expression profiling of isolated cells in collaboration with the Microarray Core. The obtained data at the Core will be put in the database, where all the Projects will have access. The primary objectives of the Sample Core are: 1) To collect blood draw samples and apheresis samples from healthy subjects including those mobilized with G-CSF. 2) To elutriate cells from blood apheresis samples to obtain lymphocyte- and monocyte-rich fractions. 3) To isolate CD34+ hematopoietic progenitor cells from G-CSF mobilized apheresis blood samples. 4) To analyze the phenotype of blood and nasal wash samples by polychromatic flow cytometry. 5) To isolate specific cells by cell sorting from blood and nasal wash samples. 6) To dispatch elutriated or isolated cells to Projects and Tech Devs. Thus, the Sample Core will be a central facility to provide appropriate types of cells and data (including phenotype and microarray data) to all the Projects and Tech Devs.

Public Health Relevance

Sample Core will be in charge of the collection, isolation of specific cells, and dispatching cell samples. The Core also will perform immunological and gene analysis of isolated cells, which will be utilized in the Projects. Thus, the Sample Core will be a central facility to provide appropriate cells and data to all the Projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI057234-10
Application #
8464007
Study Section
Special Emphasis Panel (ZAI1-KS-I)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
10
Fiscal Year
2013
Total Cost
$119,003
Indirect Cost
$34,319
Name
Baylor Research Institute
Department
Type
DUNS #
145745022
City
Dallas
State
TX
Country
United States
Zip Code
75204
Schmitt, Nathalie; Liu, Yang; Bentebibel, Salah-Eddine et al. (2016) Molecular Mechanisms Regulating T Helper 1 versus T Follicular Helper Cell Differentiation in Humans. Cell Rep 16:1082-95
Salabert, Nina; Todorova, Biliana; Martinon, Frédéric et al. (2016) Intradermal injection of an anti-Langerin-HIVGag fusion vaccine targets epidermal Langerhans cells in nonhuman primates and can be tracked in vivo. Eur J Immunol 46:689-700
Kovats, S; Turner, S; Simmons, A et al. (2016) West Nile virus-infected human dendritic cells fail to fully activate invariant natural killer T cells. Clin Exp Immunol 186:214-226
Blohmke, Christoph J; Darton, Thomas C; Jones, Claire et al. (2016) Interferon-driven alterations of the host's amino acid metabolism in the pathogenesis of typhoid fever. J Exp Med 213:1061-77
Yin, Wenjie; Gorvel, Laurent; Zurawski, Sandra et al. (2016) Functional Specialty of CD40 and Dendritic Cell Surface Lectins for Exogenous Antigen Presentation to CD8(+) and CD4(+) T Cells. EBioMedicine 5:46-58
Ueno, Hideki; Banchereau, Jacques; Vinuesa, Carola G (2015) Pathophysiology of T follicular helper cells in humans and mice. Nat Immunol 16:142-52
Schmitt, Nathalie; Ueno, Hideki (2015) Regulation of human helper T cell subset differentiation by cytokines. Curr Opin Immunol 34:130-6
Joo, HyeMee; Upchurch, Katherine; Zhang, Wei et al. (2015) Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization. J Immunol 195:1723-31
Akinbobuyi, Babatope; Byrd, Matthew R; Chang, Charles A et al. (2015) Facile syntheses of functionalized toll-like receptor 7 agonists. Tetrahedron Lett 56:458-460
Trobaugh, Derek; Green, Sharone (2015) Of Mice and Men: Protective and Pathogenic Immune Responses to West Nile virus Infection. Curr Trop Med Rep 2:41-48

Showing the most recent 10 out of 120 publications