The UCLA-CMCR Animal Core provides a full support fro animal experiments in all Full Projects and many Pilot Projects. The Core is a self-contained, fiscally independent, strict barrier facility that breeds and maintains, at a low cost, the highest possible quality gnotobiotic mice with a defined microbiological flora. It maintains breeding colonies of multiple immune competent, immune deficient, and transgenic strains that allow definitive studies on DNA repair, DNA damage response, cytokine, Toll-like receptor and other pathways that are relevant to radiation effects. In the past funding period, an average of 5,500 mice per year were used by full projects and it contributed greatly to the success of the UCLA-CMCR. The facility has an AEC cesium source 'en suite'with jigs enabling whole and partial body irradiations to be performed. X-ray and cobalt radiation machines are also available. It provides assistance with animal Irradiations on an as needed basis. It has a level II Biohazard facility and can accommodate studies on gene transfer, radioisotopes, and pathogens and provide assistance with such studies. The Core assists investigators in obtaining IACUC approval, monitors compliance and animal health, as well as providing training and advice for investigators in animal handling, procedures, the design and performance of radiation experiments and analysis and interpretation of results. This resource is available to all members of the UCLA-CMCR and others outside of UCLA on a recharge basis. These activities can be fully integrated because the facility is under the direct control of Dr. McBride who has the power to allocate space and resources to the CMCR, whose investigators receive priority. The Core brings great expertise in the responses of most normal tissues to radiation, in DNA repair, in genetics, and in immunity. It provides a variety of models to address the product potential of mitigators of radiation tissue damage and the pathways by which they may operate. It will perform a vital function in integrating the projects within the CMCR and in standardizing the information that is derived from individual animal experimentation. In so doing, it will give added value to the CMCR.

Public Health Relevance

This is a highly integrated full service Core that is able to provide a high level of quality control for investigators in the. CMCR who wish to use animal models in their research. This is possible because the facility is under the direct control ofthe P.I., who ensures that the best experimental design and methods are used and that the animals are treated humanely in full compliance with all local and national guidelines.

National Institute of Health (NIH)
Research Program--Cooperative Agreements (U19)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Los Angeles
Los Angeles
United States
Zip Code
Micewicz, Ewa D; Luong, Hai T; Jung, Chun-Ling et al. (2014) Novel dimeric Smac analogs as prospective anticancer agents. Bioorg Med Chem Lett 24:1452-7
Damoiseaux, Robert (2014) UCLA's Molecular Screening Shared Resource: enhancing small molecule discovery with functional genomics and new technology. Comb Chem High Throughput Screen 17:356-68
Erde, Jonathan; Loo, Rachel R Ogorzalek; Loo, Joseph A (2014) Enhanced FASP (eFASP) to increase proteome coverage and sample recovery for quantitative proteomic experiments. J Proteome Res 13:1885-95
Bunimovich, Yuri L; Nair-Gill, Evan; Riedinger, Mireille et al. (2014) Deoxycytidine kinase augments ATM-Mediated DNA repair and contributes to radiation resistance. PLoS One 9:e104125
Martin, N T; Nakamura, K; Paila, U et al. (2014) Homozygous mutation of MTPAP causes cellular radiosensitivity and persistent DNA double-strand breaks. Cell Death Dis 5:e1130
Hacke, K; Treger, J A; Bogan, B T et al. (2013) Genetic modification of mouse bone marrow by lentiviral vector-mediated delivery of hypoxanthine-Guanine phosphoribosyltransferase short hairpin RNA confers chemoprotection against 6-thioguanine cytotoxicity. Transplant Proc 45:2040-4
Martin, Nathan T; Nakamura, Kotoka; Davies, Robert et al. (2013) ATM-dependent MiR-335 targets CtIP and modulates the DNA damage response. PLoS Genet 9:e1003505
Xie, Michael W; Gorodetsky, Raphael; Micewicz, Ewa D et al. (2013) Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits. J Invest Dermatol 133:553-61
Ambrose, Mark; Gatti, Richard A (2013) Pathogenesis of ataxia-telangiectasia: the next generation of ATM functions. Blood 121:4036-45
Li, Xinmin; Zhou, Jian; Nahas, Shareef A et al. (2012) Common copy number variations in fifty radiosensitive cell lines. Genomics 99:96-100

Showing the most recent 10 out of 63 publications