The Immune Monitoring Core for RadCCORE is comprised of technologies and services offered by two service laboratories within the Duke Human Vaccine Institute (DHVI): Flow Cytometry Facility, and Immune Reconstitution and Biomarker Facility. The RadCCORE Immune Monitoring Core (Core C) will routinely provide investigators with high quality, state-of-the-art cell sorting, multiplex protein arrays, and T cell receptor gene expression analysis for basic and applied research efforts. Services offered in the renewal reflect actual Core C utilization in years 1-5. Alms of the Immune Monitoring Core are: 1) Provide state-of the-art, multi-color, fluorescence activated cell sorting;2) Provide targeted multiplex protein array profiling of biological samples, such as tissue culture supernatant, serum/plasma, and lung lavage fluid, using our BioPlex bead array reader (BioRad);and 3) Provide T cell immune reconstitution monitoring in mice, humans and monkeys by performing T cell receptor excision circle quantification and repertoire analysis of peripheral T cell receptor beta variable gene utilization. By educating and working in collaboration with the RadCCORE scientific program user base we will develop and optimize their specific assays and more effectively utilize the state-of-the-art instrumentation offered by the Immune Monitoring Core. Critical to all three of our aims is a commitment to ongoing development of new assays to anticipate and meet the future needs of the RadCCORE investigators.

Public Health Relevance

The RadCCORE Immune Core will provide high quality, state-of-the-art cell sorting, multiplex protein arrays, and T cell receptor gene expression analysis for their basic and applied research efforts defined in the Projects. Comprehensive and centralized immune monitoring will add value to RadCCORE studies, and will allow investigators to better and more rapidly develop the next generation of radiation countermeasures.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI067798-09
Application #
8508657
Study Section
Special Emphasis Panel (ZAI1-KS-I)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
9
Fiscal Year
2013
Total Cost
$79,090
Indirect Cost
$10,688
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Linz, Brandon M L; Neely, Crystal J; Kartchner, Laurel B et al. (2017) Innate Immune Cell Recovery Is Positively Regulated by NLRP12 during Emergency Hematopoiesis. J Immunol 198:2426-2433
Racioppi, Luigi; Lento, William; Huang, Wei et al. (2017) Calcium/calmodulin-dependent kinase kinase 2 regulates hematopoietic stem and progenitor cell regeneration. Cell Death Dis 8:e3076
Swanson, Karen V; Junkins, Robert D; Kurkjian, Cathryn J et al. (2017) A noncanonical function of cGAMP in inflammasome priming and activation. J Exp Med 214:3611-3626
Lee, Jaewoo; Jackman, Jennifer G; Kwun, Jean et al. (2017) Nucleic acid scavenging microfiber mesh inhibits trauma-induced inflammation and thrombosis. Biomaterials 120:94-102
Andrews, Rachel N; Metheny-Barlow, Linda J; Peiffer, Ann M et al. (2017) Cerebrovascular Remodeling and Neuroinflammation is a Late Effect of Radiation-Induced Brain Injury in Non-Human Primates. Radiat Res 187:599-611
Chen, Liang; Wilson, Justin E; Koenigsknecht, Mark J et al. (2017) NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth. Nat Immunol 18:541-551
Himburg, Heather A; Doan, Phuong L; Quarmyne, Mamle et al. (2017) Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med 23:91-99
Jha, Sushmita; Brickey, W June; Ting, Jenny Pan-Yun (2017) Inflammasomes in Myeloid Cells: Warriors Within. Microbiol Spectr 5:
DeBo, Ryne J; Lees, Cynthia J; Dugan, Greg O et al. (2016) Late Effects of Total-Body Gamma Irradiation on Cardiac Structure and Function in Male Rhesus Macaques. Radiat Res 186:55-64
Mont, Stacey; Davies, Sean S; Roberts Second, L Jackson et al. (2016) Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919

Showing the most recent 10 out of 188 publications