Abstract

Ionizing irradiation induces RNAs in cells which can be targeted prior to irradiation by siRNA to identify novel targets for radioprotector drugs. We have successfully used this strategy to identify a co,mmon drug, Glyburide, as a novel radioprotector. We now propose to apply this strategy to identify radiation mitigator drugs using radiosensitive human cell targets and siRNA screening after irradiation. The 1** Specific Aim will optimize and deploy sentinel cells for detecting RNA targets for new mitigator drugs.
The second aim will be to use druggable siRNA libraries and small molecule libraries to identify radiation damage mitigators.
The third aim will be to identify targets that interact positively with prioritized drugs to advance radiation mitigation effects.

Public Health Relevance

We have successfully proven that siRNA library screening can be used to identify radioprotector drugs among commonly available pharmacologic agents. This same strategy will identify mitigator drugs that may be available in the existing pharmacopea and their interaction with prioritized compounds developed already in the CMCR..

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI068021-09
Application #
8515296
Study Section
Special Emphasis Panel (ZAI1-KS-I)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
9
Fiscal Year
2013
Total Cost
$401,176
Indirect Cost
$121,700

  Institution

Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Hassannia, Behrouz; Wiernicki, Bartosz; Ingold, Irina et al. (2018) Nano-targeted induction of dual ferroptotic mechanisms eradicates high-risk neuroblastoma. J Clin Invest 128:3341-3355
Conrad, Marcus; Kagan, Valerian E; Bayir, Hülya et al. (2018) Regulation of lipid peroxidation and ferroptosis in diverse species. Genes Dev 32:602-619
Stoyanovsky, Anastas D; Stoyanovsky, Detcho A (2018) 1-Oxo-2,2,6,6-tetramethylpiperidinium bromide converts ?-H N,N-dialkylhydroxylamines to nitrones via a two-electron oxidation mechanism. Sci Rep 8:15323
Zhou, Shuanhu; Glowacki, Julie (2018) Dehydroepiandrosterone and Bone. Vitam Horm 108:251-271
Robinson, Andria R; Yousefzadeh, Matthew J; Rozgaja, Tania A et al. (2018) Spontaneous DNA damage to the nuclear genome promotes senescence, redox imbalance and aging. Redox Biol 17:259-273
Gaschler, Michael M; Andia, Alexander A; Liu, Hengrui et al. (2018) FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol 14:507-515
Tyurina, Yulia Y; Shrivastava, Indira; Tyurin, Vladimir A et al. (2018) ""Only a Life Lived for Others Is Worth Living"": Redox Signaling by Oxygenated Phospholipids in Cell Fate Decisions. Antioxid Redox Signal 29:1333-1358
Schlattner, Uwe; Tokarska-Schlattner, Malgorzata; Epand, Richard M et al. (2018) NME4/nucleoside diphosphate kinase D in cardiolipin signaling and mitophagy. Lab Invest 98:228-232
Willis, John; Epperly, Michael W; Fisher, Renee et al. (2018) Amelioration of Head and Neck Radiation-Induced Mucositis and Distant Marrow Suppression in Fanca-/- and Fancg-/- Mice by Intraoral Administration of GS-Nitroxide (JP4-039). Radiat Res 189:560-578
Leibowitz, Brian J; Yang, Liheng; Wei, Liang et al. (2018) Targeting p53-dependent stem cell loss for intestinal chemoprotection. Sci Transl Med 10:

Showing the most recent 10 out of 203 publications