The TRIAD Toolkit Core will offer the TRIAD team expertly guided access to a wide range of publically available and proprietary tools for genome screening and protein characterization. The TRIAD toolkit team will: establish a consultancy relationship with program team leaders and staff;provide awareness, education and support services for program designers and team leaders;provide hands-on training and support to the TRIAD program teams in the use of publically available and proprietary tools related to the fields of Genomics, Proteomics, Bioinformatics, and Computational Biology;and provide education, training and support related to the interpretation of analytical results produced through the use of the TRIAD Toolkit. All of the tools that have been developed by EpiVax for the purposes of vaccine design will be available to the TRIAD investigators for the purposes of this RFA. For example, the EpiMatrix algorithm will be available to screen genomic sequences from TRIAD project genomes for T-cell epitope content. The ClustiMer algorithm will be used to identify clusters of Class II T-helper epitopes contained in short protein segments. The Conservatrix algorithm will be used to select putative epitopes that are conserved across a range of variant protein isolates for even the most mutable of protein targets. Conserved sequences will be knit together into highly immunogenic consensus sequences, using an algorithm known as EpiAssembler. In order to be able to find the minimal set of epitopes that """"""""covers"""""""" the maximum number of HLA-types and observed strains of the target pathogen, Aggregatrix will be available. VaccineCAD will be used to convert any epitope set as input material and create an optimized string-of-beads while minimizing deleterious """"""""junctional"""""""" epitopes. The TRIAD Toolkit team will also provide access to high throughput HLA binding assays. By the end of the fiveyear program, the TRIAD toolkit team will have created a selected set .of user-friendly vaccine design tools on the TRIAD Toolkit website, which will be made available (following registration, to enable tracking) for public use. TRIAD investigators will have become informed users of the TRIAD toolkit, and researchers who are new to the field of human immunology will be engaged in discovery projects using the toolkit.

Public Health Relevance

The Toolkit core will enable TRIAD to accelerate the development of vaccines for emerging and biowarfare pathogens and educate new generations of vaccine researchers, leading to significant improvements in global health and a radical transformation in the approach to vaccine design around the world.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI082642-05
Application #
8501263
Study Section
Special Emphasis Panel (ZAI1-KS-I)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$207,065
Indirect Cost
$44,163
Name
University of Rhode Island
Department
Type
DUNS #
144017188
City
Kingston
State
RI
Country
United States
Zip Code
02881
Tomimaru, Yoshito; Mishra, Sasmita; Safran, Howard et al. (2015) Aspartate-β-hydroxylase induces epitope-specific T cell responses in hepatocellular carcinoma. Vaccine 33:1256-66
Terry, Frances E; Moise, Leonard; Martin, Rebecca F et al. (2015) Time for T? Immunoinformatics addresses vaccine design for neglected tropical and emerging infectious diseases. Expert Rev Vaccines 14:21-35
Liu, Rui; Moise, Leonard; Tassone, Ryan et al. (2015) H7N9 T-cell epitopes that mimic human sequences are less immunogenic and may induce Treg-mediated tolerance. Hum Vaccin Immunother 11:2241-52
Becker, Martin; Felsberger, André; Frenzel, André et al. (2015) Application of M13 phage display for identifying immunogenic proteins from tick (Ixodes scapularis) saliva. BMC Biotechnol 15:43
Losikoff, Phyllis T; Mishra, Sasmita; Terry, Frances et al. (2015) HCV epitope, homologous to multiple human protein sequences, induces a regulatory T cell response in infected patients. J Hepatol 62:48-55
De Groot, Anne S; Ross, Ted M; Levitz, Lauren et al. (2015) C3d adjuvant effects are mediated through the activation of C3d-specific autoreactive T cells. Immunol Cell Biol 93:189-97
Eickhoff, Christopher S; Van Aartsen, Daniel; Terry, Frances E et al. (2015) An immunoinformatic approach for identification of Trypanosoma cruzi HLA-A2-restricted CD8(+) T cell epitopes. Hum Vaccin Immunother 11:2322-8
Pichu, Sivakamasundari; Ribeiro, Jose M C; Mather, Thomas N et al. (2014) Purification of a serine protease and evidence for a protein C activator from the saliva of the tick, Ixodes scapularis. Toxicon 77:32-9
Shattuck, Wendy M C; Dyer, Megan C; Desrosiers, Joe et al. (2014) Partial pathogen protection by tick-bite sensitization and epitope recognition in peptide-immunized HLA DR3 transgenic mice. Hum Vaccin Immunother 10:3048-59
Zhang, Songhua; Desrosiers, Joseph; Aponte-Pieras, Jose R et al. (2014) Human immune responses to H. pylori HLA Class II epitopes identified by immunoinformatic methods. PLoS One 9:e94974

Showing the most recent 10 out of 43 publications