The overall objective of the Clinical Core is to provide human specimens for the studies described in Research Projects 1-3. These specimens will supplement the existing extensive collection of unique banked specimens that are being used and accrued in the current CCHI. Note: the core will only obtain human specimens from subjects who are receiving the standard-of-care (e.g., vaccinated with the Ty21a typhoid vaccine because of travel to endemic areas or work in microbiology labs, or undergoing medically-indicated colonoscopies) and do not involve off-label interventions. The goals of this core will be achieved through the following specific aims.

Public Health Relevance

The availability of human specimens will be a crucial resource for the three Research Projects (RPI-3) and their goals to comprehensively study the local and systemic immune response to vaccination, evaluate the role of the gut microbiome, and measure the role of the micro-milieu in response to S. Typhi. The Clinical Core will support the interrelated, complementary, interactive goals of RPI-3.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
2U19AI082655-06
Application #
8726153
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Maryland Baltimore
Department
Type
DUNS #
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Salerno-Goncalves, R; Safavie, F; Fasano, A et al. (2016) Free and complexed-secretory immunoglobulin A triggers distinct intestinal epithelial cell responses. Clin Exp Immunol 185:338-47
Fresnay, Stephanie; McArthur, Monica A; Magder, Laurence et al. (2016) Salmonella Typhi-specific multifunctional CD8+ T cells play a dominant role in protection from typhoid fever in humans. J Transl Med 14:62
Blohmke, Christoph J; Darton, Thomas C; Jones, Claire et al. (2016) Interferon-driven alterations of the host's amino acid metabolism in the pathogenesis of typhoid fever. J Exp Med 213:1061-77
Salerno-Goncalves, Rosangela; Fasano, Alessio; Sztein, Marcelo B (2016) Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity. J Vis Exp :
McArthur, Monica A; Fresnay, Stephanie; Magder, Laurence S et al. (2015) Activation of Salmonella Typhi-specific regulatory T cells in typhoid disease in a wild-type S. Typhi challenge model. PLoS Pathog 11:e1004914
Trebicka, Estela; Shanmugam, Nanda Kumar N; Chen, Kejie et al. (2015) Intestinal Inflammation Leads to a Long-lasting Increase in Resistance to Systemic Salmonellosis that Requires Macrophages But Not B or T Lymphocytes at the Time of Pathogen Challenge. Inflamm Bowel Dis 21:2758-65
Wahid, R; Fresnay, S; Levine, M M et al. (2015) Immunization with Ty21a live oral typhoid vaccine elicits crossreactive multifunctional CD8+ T-cell responses against Salmonella enterica serovar Typhi, S. Paratyphi A, and S. Paratyphi B in humans. Mucosal Immunol 8:1349-59
Booth, Jayaum S; Salerno-Goncalves, Rosangela; Blanchard, Thomas G et al. (2015) Mucosal-Associated Invariant T Cells in the Human Gastric Mucosa and Blood: Role in Helicobacter pylori Infection. Front Immunol 6:466
Toapanta, Franklin R; Bernal, Paula J; Fresnay, Stephanie et al. (2015) Oral Wild-Type Salmonella Typhi Challenge Induces Activation of Circulating Monocytes and Dendritic Cells in Individuals Who Develop Typhoid Disease. PLoS Negl Trop Dis 9:e0003837
Sztein, Marcelo B; Salerno-Goncalves, Rosangela; McArthur, Monica A (2014) Complex adaptive immunity to enteric fevers in humans: lessons learned and the path forward. Front Immunol 5:516

Showing the most recent 10 out of 41 publications