This core will provide services to University of Chicago Autoimmunity Center of Excellence (UCACE) investigators to characterize the role of B lymphocytes in autoimmune pathology. There are two state-of-the-art technologies that will allow the center investigators to scrutinize autoantibody-mediated pathology. 1. Analysis of B lymphocyte specificity using recombinant monoclonal (mAb) technology. 2. Immunoglobulin light chain variable gene microarrays Finally, Drs. Weigert and Wilson will assist and provide technical support for studies of the immunoglobulin repertoire using single-cell PCR, cloning and sequencing. This approach will augment the use of light chain microarrays and human mAb analysis. The unique methodologies for scrutinizing human B cell mediated pathology will provide powerful tools to the center to facilitate our understanding of lupus and myasthenia gravis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI082724-05
Application #
8477117
Study Section
Special Emphasis Panel (ZAI1-QV-I)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
5
Fiscal Year
2013
Total Cost
$199,866
Indirect Cost
$71,746
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
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Steinsbø, Øyvind; Henry Dunand, Carole J; Huang, Min et al. (2014) Restricted VH/VL usage and limited mutations in gluten-specific IgA of coeliac disease lesion plasma cells. Nat Commun 5:4041
Chang, Anthony; Ko, Kichul; Clark, Marcus R (2014) The emerging role of the inflammasome in kidney diseases. Curr Opin Nephrol Hypertens 23:204-10
Kinloch, Andrew J; Chang, Anthony; Ko, Kichul et al. (2014) Vimentin is a dominant target of in situ humoral immunity in human lupus tubulointerstitial nephritis. Arthritis Rheumatol 66:3359-70
Iversen, Rasmus; Di Niro, Roberto; Stamnaes, Jorunn et al. (2013) Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91
Andrews, Sarah F; Zhang, Qingzhao; Lim, Samuel et al. (2013) Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity. J Exp Med 210:125-42
Di Niro, Roberto; Mesin, Luka; Zheng, Nai-Ying et al. (2012) High abundance of plasma cells secreting transglutaminase 2-specific IgA autoantibodies with limited somatic hypermutation in celiac disease intestinal lesions. Nat Med 18:441-5
Hsieh, Christine; Chang, Anthony; Brandt, Daniel et al. (2011) Predicting outcomes of lupus nephritis with tubulointerstitial inflammation and scarring. Arthritis Care Res (Hoboken) 63:865-74
Chang, Anthony; Henderson, Scott G; Brandt, Daniel et al. (2011) In situ B cell-mediated immune responses and tubulointerstitial inflammation in human lupus nephritis. J Immunol 186:1849-60
Kaur, Kaval; Sullivan, Meghan; Wilson, Patrick C (2011) Targeting B cell responses in universal influenza vaccine design. Trends Immunol 32:524-31

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