The mission of this Core is to provide state-of-art imaging technology and services to support the study: """"""""Host-pathogen competition in IFN mediated antiviral defense."""""""" The proposed study incorporates skilled resources from more than four institutions addressing different but focused research questions involved in understanding the control and regulation of RIG-I- and MDA5-mediated immune surveillance during viral infection, with the overarching goal of developing novel therapeutic strategies for virus-associated disorders. As such, the Core currently features two major imaging systems: a spectral imaging confocal laser scanning microscope system equipped with live-cell imaging observation and a super-resolution molecule imaging system. All five projects will rely heavily on this Core, co-directed by Drs. Liang and Myong. What follows is a description of the confocal live-cell imaging portion of the Core followed by a description of the super-resolution single molecule microscopy services provided by the Core. All data from both systems are accessible to every PI. The data will be uploaded in the Center's website for data sharing and data mining. The amount of time supported varies over the 5-year course. The super-imaging system is more required in Projects 3 and 5, while the confocal system mainly supports Projects 1, 3, 4, and 5. In addition, the Imaging Core will coordinate the scientific and technologic services provided by each project.

Public Health Relevance

The key to understand a protein's function is the accurate determination of its spatial/temporal distribution inside cells and its association with other relevant molecules. Using advanced technology provided by the imaging core to characterize RIG-1 performance will greatly facilitate our understanding of RIG-I during viral infection, thereby providing important insights for virus-infected diseases'control and theraphy.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI083025-04
Application #
8378254
Study Section
Special Emphasis Panel (ZAI1-BDP-I)
Project Start
Project End
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
4
Fiscal Year
2012
Total Cost
$143,032
Indirect Cost
$26,314
Name
University of Southern California
Department
Type
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
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