Artemisinin-based combination therapies (ACTs) play an essential role in the current malaria control and elimination campaign. However, the circulation of counterfeit and substandard artemisinins greatly threatens the malaria control efforts. Fake artemisinins containing no or little active ingredients are life-threatening, while substandard drugs encourage resistance development. The scale of this 'lethal problem'is particularly huge in some Southeast Asian countries, where as much as 64% of the artesunate sold on the market was fake. To prolong the life span of artemisinin drugs for treating malaria, strict drug quality control needs to be implemented in these nations. For this purpose, fast and reliable methods of artemisinin detection and quantitation are needed. Traditional methods for quantitation of artemisinins often require expensive instruments and substantial technical support, whereas the rapid qualitative method for screening of fake artemisinin derivatives is less sensitive and variable. Based on our recent success of developing a highly sensitive and reliable enzyme-linked immunosorbent assay (ELISA) using a specific monoclonal antibody against artemisinins, we propose to 1) develop a series of monoclonal antibodies with specificities for the major artemisinin derivatives (dihydroartemisinin, artemether, arteether, and artesunate) using an innovative design of immunogens, 2) develop and optimize an ELISA for accurate quantification of artemisinins, and a lateral flow dipstick assay for rapid, semi-quantitative analysis of artemisinins in antimalarial drugs, 3) perform systematic field surveys of the drug quality of artemisinin-based antimalarial medicines in three Southeast Asian countries using these new tools, and 4) optimize the ELISA conditions so that it can be used as an alternative tool for quantification of artemisinins and its derivatives in pharmacokinetic studies. These immunoassays will offer convenient tools for both accurate quantification of artemisinins and rapid detection of counterfeit and substandard artemisinin derivative drugs, contributing to antimalarial drug quality control.
The prevalence of counterfeit and substandard artemisinin drugs is a big threat to the success of the malaria control campaign. In response to this escalating problem, we want to design two convenient, monoclonal antibody-based immunoassays for accurate quantification of artemisinin derivatives in laboratories and rapid, semi-quantitative analysis of artemisinins in antimalarial drugs under field conditions.
|Wang, Meilian; Siddiqui, Faiza Amber; Fan, Qi et al. (2016) Limited genetic diversity in the PvK12 Kelch protein in Plasmodium vivax isolates from Southeast Asia. Malar J 15:537|
|Liu, Huaie; Feng, Guohua; Zeng, Weilin et al. (2016) A more appropriate white blood cell count for estimating malaria parasite density in Plasmodium vivax patients in northeastern Myanmar. Acta Trop 156:152-6|
|Zhu, Xiaotong; Zhao, Zhenjun; Feng, Yonghui et al. (2016) Genetic diversity of the Plasmodium falciparum apical membrane antigen I gene in parasite population from the China-Myanmar border area. Infect Genet Evol 39:155-62|
|Wang, Qinghui; Zhao, Zhenjun; Zhang, Xuexing et al. (2016) Naturally Acquired Antibody Responses to Plasmodium vivax and Plasmodium falciparum Merozoite Surface Protein 1 (MSP1) C-Terminal 19 kDa Domains in an Area of Unstable Malaria Transmission in Southeast Asia. PLoS One 11:e0151900|
|Wang, Xiaoming; Zhou, Guofa; Zhong, Daibin et al. (2016) Life-table studies revealed significant effects of deforestation on the development and survivorship of Anopheles minimus larvae. Parasit Vectors 9:323|
|MÃ©nard, Didier; Khim, Nimol; Beghain, Johann et al. (2016) A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms. N Engl J Med 374:2453-64|
|Hu, Yue; Zhou, Guofa; Ruan, Yonghua et al. (2016) Seasonal dynamics and microgeographical spatial heterogeneity of malaria along the China-Myanmar border. Acta Trop 157:12-9|
|Guo, Suqin; Zhang, Wei; He, Lishan et al. (2016) Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick. Anal Bioanal Chem 408:6003-8|
|Xu, Xin; Zhou, Guofa; Wang, Ying et al. (2016) Microgeographic Heterogeneity of Border Malaria During Elimination Phase, Yunnan Province, China, 2011-2013. Emerg Infect Dis 22:1363-70|
|Guo, Suqin; He, Lishan; Tisch, Daniel J et al. (2016) Pilot testing of dipsticks as point-of-care assays for rapid diagnosis of poor-quality artemisinin drugs in endemic settings. Trop Med Health 44:15|
Showing the most recent 10 out of 88 publications