People living in areas where Plasmodium falciparum (Pf) and P. vivax (Pv) are endemic develop naturally acquired immunity to malaria blood stage infection and illness after repeated exposures to the parasite through childhood and adolescence. The rate at which this immunity develops is variable due to local differences in the intensity and temporal stability of transmission. A global effort is now underway to control and ultimately eliminate Pf and Pv through the use of vector interventions such as insecticidal bed nets. The goal of research proposed here is to identify novel and robust antibody assays to guide this effort. Probing a protein microarray containing 2,320 /^proteins with sera collected from Pf-exposed Malian children and adults indicated that long-lived antibody responses against hundreds of antigens are acquired after years of repeated /^infections and antibodies associated with protection were identified against 49 specific Pf antigens.^ Here we aim to extend these findings with a wortdwide sero-epidemiological analysis. This multi- ICEMR project will probe specimens from cross sectional and longitudinal studies in 5 regions around the world which differ in malaria transmission intensity and in Plasmodium species exposure. The array will contain 800 antigens from fYand Pv, and sera from the studies will be probed annually to determine the effect of vector control and chemoprevention on the development and decline ofthe serological response. This study will better our understanding of how naturally acquired immunity develops as a function of age and seasonal mosquito exposure. The data may also lead to the discovery of antibody biomarkers associated with parasite exposure and with protection from malarial disease.

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The study will determine antibody responses against 500 Pf and Pv antigens in cross sectional specimens collected from 5 different ICEMR locations around the wortd. To make results comparable between sites, harmonized cross sectional studies from the different sites will be age stratified between 2-25 years of age, and taken at 2 time points before and after the high transmission season for each location. Analysis of the specimens will also be harmonized by coordinating protein microarray probing and analysis through the Protein Microarray Laboratory at UC Irvine.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1-AWA-M (M1))
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Case Western Reserve University
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Nicolete, Vanessa C; Frischmann, Sarah; Barbosa, Susana et al. (2016) Naturally Acquired Binding-Inhibitory Antibodies to Plasmodium vivax Duffy Binding Protein and Clinical Immunity to Malaria in Rural Amazonians. J Infect Dis 214:1539-1546
Echeverry, Diego F; Deason, Nicholas A; Davidson, Jenna et al. (2016) Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene. Malar J 15:128
Small, Scott T; Reimer, Lisa J; Tisch, Daniel J et al. (2016) Population genomics of the filarial nematode parasite Wuchereria bancrofti from mosquitoes. Mol Ecol 25:1465-77
Logue, Kyle; Keven, John Bosco; Cannon, Matthew V et al. (2016) Unbiased Characterization of Anopheles Mosquito Blood Meals by Targeted High-Throughput Sequencing. PLoS Negl Trop Dis 10:e0004512
Russell, Tanya L; Beebe, Nigel W; Bugoro, Hugo et al. (2016) Determinants of host feeding success by Anopheles farauti. Malar J 15:152
Russell, Tanya L; Beebe, Nigel W; Bugoro, Hugo et al. (2016) Anopheles farauti is a homogeneous population that blood feeds early and outdoors in the Solomon Islands. Malar J 15:151
Russell, Tanya L; Beebe, Nigel W; Bugoro, Hugo et al. (2016) Frequent blood feeding enables insecticide-treated nets to reduce transmission by mosquitoes that bite predominately outdoors. Malar J 15:156
Guo, Suqin; He, Lishan; Tisch, Daniel J et al. (2016) Pilot testing of dipsticks as point-of-care assays for rapid diagnosis of poor-quality artemisinin drugs in endemic settings. Trop Med Health 44:15
Russell, Tanya L; Burkot, Thomas R; Bugoro, Hugo et al. (2016) Larval habitats of the Anopheles farauti and Anopheles lungae complexes in the Solomon Islands. Malar J 15:164
Jain, Aarti; Taghavian, Omid; Vallejo, Derek et al. (2016) Evaluation of quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays. Proteomics 16:1271-9

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