The Shared Technology Cores in the USA and India will provide services to multiple projects of this ICEMR grant. These services include sequencing, genotyping of multiple organisms (including humans, parasites, and mosquitoes), microarray infrastructure and analysis, and microfluidic fabrication at The Center Genomic Applications (TCGA) and the University of Washington (UW).
TO OVERALL APPLICATION: Due to the reliance of all projects on the isolation and characterization of parasites exhibiting the Accelerated Resistance to Multiple Drugs (ARMD) phenotype, genotyping facilities will be of central importance. In addition, central aims of Project 3 depend on the fabrication of microfluidic devices to assess erythrocyte deformability following Plasmodium Invasion. Lastly, mosquito transcriptomics in Project 4 and genome-wide association studies in Project 5 will require extensive microarray infrastructure provided by this core.
|Deng, Xiaoyi; Kokkonda, Sreekanth; El Mazouni, Farah et al. (2014) Fluorine modulates species selectivity in the triazolopyrimidine class of Plasmodium falciparum dihydroorotate dehydrogenase inhibitors. J Med Chem 57:5381-94|
|Rice, Benjamin L; Acosta, Mónica M; Pacheco, M Andreína et al. (2014) The origin and diversification of the merozoite surface protein 3 (msp3) multi-gene family in Plasmodium vivax and related parasites. Mol Phylogenet Evol 78:172-84|
|Turner, Louise; Lavstsen, Thomas; Berger, Sanne S et al. (2013) Severe malaria is associated with parasite binding to endothelial protein C receptor. Nature 498:502-5|
|Herricks, Thurston; Avril, Marion; Janes, Joel et al. (2013) Clonal variants of Plasmodium falciparum exhibit a narrow range of rolling velocities to host receptor CD36 under dynamic flow conditions. Eukaryot Cell 12:1490-8|
|Guler, Jennifer L; Freeman, Daniel L; Ahyong, Vida et al. (2013) Asexual populations of the human malaria parasite, Plasmodium falciparum, use a two-step genomic strategy to acquire accurate, beneficial DNA amplifications. PLoS Pathog 9:e1003375|
|Kumar, Ashwani; Chery, Laura; Biswas, Chinmoy et al. (2012) Malaria in South Asia: prevalence and control. Acta Trop 121:246-55|
|Tutterrow, Yeung L; Avril, Marion; Singh, Kavita et al. (2012) High levels of antibodies to multiple domains and strains of VAR2CSA correlate with the absence of placental malaria in Cameroonian women living in an area of high Plasmodium falciparum transmission. Infect Immun 80:1479-90|
|Narayanasamy, Krishnamoorthy; Chery, Laura; Basu, Analabha et al. (2012) Malaria evolution in South Asia: knowledge for control and elimination. Acta Trop 121:256-66|
|Herricks, Thurston; Seydel, Karl B; Turner, George et al. (2011) A microfluidic system to study cytoadhesion of Plasmodium falciparum infected erythrocytes to primary brain microvascularendothelial cells. Lab Chip 11:2994-3000|
|Gujjar, Ramesh; El Mazouni, Farah; White, Karen L et al. (2011) Lead optimization of aryl and aralkyl amine-based triazolopyrimidine inhibitors of Plasmodium falciparum dihydroorotate dehydrogenase with antimalarial activity in mice. J Med Chem 54:3935-49|