Highly multiplexed and quantitative gene expression assays, capable of precisely measuring hundreds of transcripts provide a robust approach with broad applicability for monitoring the human immune system. There is a strong demand for technologies which bridge the gap between microarrays, which are good for broad surveys of thousands of genes, but are weak on quantitation, and PCR methods which are strong on quantitation, but have limited multiplexing capability. We will create a unique infrastructure/resource designed to accelerate scientific progress in the field of immune monitoring. We will develop biological content and establish the nCounter digital gene expression system from NanoString as a preferred platform. We will apply the platform to samples provided from investigators under the U19 project in vaccination and response to infection. The core will be available for other studies as well. The core will generate synergy and foster interactions among multi and interdisciplinary research teams that will advance public health and health care delivery.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI089992-05
Application #
8868324
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
5
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Yale University
Department
Type
DUNS #
City
New Haven
State
CT
Country
United States
Zip Code
Yao, Yi; Montgomery, Ruth R (2016) Role of Immune Aging in Susceptibility to West Nile Virus. Methods Mol Biol 1435:235-47
Montgomery, Ruth R (2016) High standards for high dimensional investigations. Cytometry A 89:886-888
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Mohanty, Subhasis; Joshi, Samit R; Ueda, Ikuyo et al. (2015) Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults. J Infect Dis 211:1174-84
Qian, Feng; Goel, Gautam; Meng, Hailong et al. (2015) Systems immunology reveals markers of susceptibility to West Nile virus infection. Clin Vaccine Immunol 22:6-16
Qian, Feng; Montgomery, Ruth R (2015) Imaging Immunosenescence. Methods Mol Biol 1343:97-106
Thakar, Juilee; Mohanty, Subhasis; West, A Phillip et al. (2015) Aging-dependent alterations in gene expression and a mitochondrial signature of responsiveness to human influenza vaccination. Aging (Albany NY) 7:38-52
Tsioris, Konstantinos; Gupta, Namita T; Ogunniyi, Adebola O et al. (2015) Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing. Integr Biol (Camb) 7:1587-97

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