The objectives ofthe Dartmouth University U19 CMCR program are to develop field biodosimetry techniques that are based on ex vivo and in vivo electron paramagnetic resonance (EPR) spectrometry measurements of a radiation-induced signal (RIS) in finger/toe nails (""""""""nails"""""""") or teeth. In this project (2), the ex vivo EPR method for measuring nails is further developed towards a field deployable application. To achieve this goal, four objectives have been deflned that address signal characterization, sample treatment and signal deconvolution to remove interfering signals, validation of test methods, identification and characterization of factors that have the potential to impact the variability of the RIS signal, and establish methods for data acquisition, processing and output suitable for use by non-expert operators and ER managers under field conditions. Characterization ofthe overall signal, the spectral components and pursuing a systematic investigation of the molecular basis of the EPR signals in both unirradiated and irradiated nails is important towards developing approaches for separating the radiation-induced signal from a mechanically-induced signal (MIS). Some aspects of these studies will utilize the data from Project 3, which will provide EPR spectra of nails without clipping, and therefore help to differentiate more clearly the origin and nature of the signals from the clipping process. The result of this set of studies is the development of a basis set of spectral components ofthe RIS an MIS signals, coupled with the decay kinetics ofthe separate component signals that are integrated into a spectrum deconvolution procedure for extracting the RIS component signal. Variability in RIS and MIS signals are to be characterized with respect to nail water content, demographics (i.e. age, race), systemic variables (pregnancy, diet/supplements, nail disorders) and fungal infection. Finally, delineating a field-usable method for sample procurement, placement ofthe samples into the spectrometer, the analytic software to determine the dose, and delineation of factors that if present need to be considered in the calibration of the results. While the construction of a field-deployable prototype that meets the overall goals ofthe Center will be carried out in collaboration with the instrumental core at Dartmouth, this project will develop the methodology to be implemented in the development and construction.

Public Health Relevance

The objectives of the project provide the supporting data and method development necessary for fielding a biodosimeter that is based on measuring a dose dependent radiation induced signal in finger or toe nail clippings.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1-KS-I)
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Dartmouth College
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Camarata, Andrew S; Switchenko, Jeffrey M; Demidenko, Eugene et al. (2016) Emesis as a Screening Diagnostic for Low Dose Rate (LDR) Total Body Radiation Exposure. Health Phys 110:391-4
Flood, Ann Barry; Ali, Arif N; Boyle, Holly K et al. (2016) Evaluating the Special Needs of The Military for Radiation Biodosimetry for Tactical Warfare Against Deployed Troops: Comparing Military to Civilian Needs for Biodosimetry Methods. Health Phys 111:169-82
Sholom, S; McKeever, S W S (2016) Emergency EPR dosimetry technique using vacuum-stored dry nails. Radiat Meas 88:41-47
Guy, Mallory L; Zhu, Lihuang; Ramanathan, Chandrasekhar (2015) Design and characterization of a W-band system for modulated DNP experiments. J Magn Reson 261:11-8
Khailov, A M; Ivannikov, A I; Skvortsov, V G et al. (2015) Calculation of dose conversion factors for doses in the fingernails to organ doses at external gamma irradiation in air. Radiat Meas 82:1-7
Woflson, Helen; Ahmad, Rizwan; Twig, Ygal et al. (2015) A magnetic resonance probehead for evaluating the level of ionizing radiation absorbed in human teeth. Health Phys 108:326-35
Desmet, Céline M; Djurkin, Andrej; Dos Santos-Goncalvez, Ana Maria et al. (2015) Tooth Retrospective Dosimetry Using Electron Paramagnetic Resonance: Influence of Irradiated Dental Composites. PLoS One 10:e0131913
Rychert, Kevin M; Zhu, Gang; Kmiec, Maciej M et al. (2015) Imaging tooth enamel using zero echo time (ZTE) magnetic resonance imaging. Proc SPIE Int Soc Opt Eng 9417:
Rogan, Peter K; Li, Yanxin; Wickramasinghe, Asanka et al. (2014) Automating dicentric chromosome detection from cytogenetic biodosimetry data. Radiat Prot Dosimetry 159:95-104
Guinan, Eva C; Palmer, Christine D; Mancuso, Christy J et al. (2014) Identification of single nucleotide polymorphisms in hematopoietic cell transplant patients affecting early recognition of, and response to, endotoxin. Innate Immun 20:697-711

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