Discovery and Target validation Proteomics (Core D) will provide the four projects of this Program Project a comprehensive proteomics analysis facility that will assist the investigators in the biomarker discovery and validation analysis required for the investigators to analyze the role of stem cells in improving the outcome of radiation-induced gastrointestinal syndrome (RIGS), Radiafion-induced hematopoietic syndrome (RIHS), and Radiafion-induced cutaneous syndrome (RICS). The core facility will provide advice on sample preparation and services for protein processing for 2D LC-MS/MS analysis. The Core will provide all necessary services for biomarker identification and validation. Services offered by the core will include: protocol design for harvesting conditioned media and irradiated tissues, protein extraction and processing of these samples for 2D LC-MS/MS analysis, performance of MudPIT analysis, database search for protein identification and quantification, and data mining. The key personnel of the core will be the core director, technical director, and one technical staff. The core director will be responsible for supervising all the activities ofthe core, offering advice on different methods and procedures, interpretation ofthe results, and generating proteomic results. The technical director (consultant) will train and supervise the technical personnel to perform 2D LC-MS/MS runs. The technical staff will prepare and process samples for proteomic analysis and perform 2D LC-MS/MS runs to collect data.

Public Health Relevance

Appropriate sample preparation and processing (protein extraction and limited proteolysis) are essential to all projects in this Program Project as they allow the study of the in vivo impact of stem cell technologies to mitigate the injuries induced by radiation exposure.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI091175-05
Application #
8881710
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
5
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
City
Bronx
State
NY
Country
United States
Zip Code
11794
Nie, Wenna; Yan, Leyu; Lee, Yie H et al. (2016) Advanced mass spectrometry-based multi-omics technologies for exploring the pathogenesis of hepatocellular carcinoma. Mass Spectrom Rev 35:331-49
Kulkarni, Shilpa; Koller, Antonius; Mani, Kartik M et al. (2016) Identifying Urinary and Serum Exosome Biomarkers for Radiation Exposure Using a Data Dependent Acquisition and SWATH-MS Combined Workflow. Int J Radiat Oncol Biol Phys 96:566-77
Brodin, N Patrik; Chen, Yong; Yaparpalvi, Ravindra et al. (2016) Dosimetry Formalism and Implementation of a Homogenous Irradiation Protocol to Improve the Accuracy of Small Animal Whole-Body Irradiation Using a 137Cs Irradiator. Health Phys 110:S26-38
Qiu, Yunping; Moir, Robyn; Willis, Ian et al. (2016) Isotopic Ratio Outlier Analysis of the S. cerevisiae Metabolome Using Accurate Mass Gas Chromatography/Time-of-Flight Mass Spectrometry: A New Method for Discovery. Anal Chem 88:2747-54
Saha, Subhrajit; Aranda, Evelyn; Hayakawa, Yoku et al. (2016) Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury. Nat Commun 7:13096
Goldman, Devorah C; Alexeev, Vitali; Lash, Elizabeth et al. (2015) The triterpenoid RTA 408 is a robust mitigator of hematopoietic acute radiation syndrome in mice. Radiat Res 183:338-44
Acharya, Sanket S; Fendler, Wojciech; Watson, Jacqueline et al. (2015) Serum microRNAs are early indicators of survival after radiation-induced hematopoietic injury. Sci Transl Med 7:287ra69
Landis, Charles S; Zhou, Hongchao; Liu, Laibin et al. (2015) Liver regeneration and energetic changes in rats following hepatic radiation therapy and hepatocyte transplantation by ³¹P MRSI. Liver Int 35:1145-51
Kurland, Irwin Jack; Broin, Pilib Ó; Golden, Aaron et al. (2015) Integrative Metabolic Signatures for Hepatic Radiation Injury. PLoS One 10:e0124795
Ó Broin, Pilib; Vaitheesvaran, Bhavapriya; Saha, Subhrajit et al. (2015) Intestinal microbiota-derived metabolomic blood plasma markers for prior radiation injury. Int J Radiat Oncol Biol Phys 91:360-7

Showing the most recent 10 out of 27 publications