Genomics technology has paved the way for a global and unbiased quantitative and qualitative assessment of the immune response. We have developed an Immune Array platform based on whole genome microarray analysis and a transcriptomic approach that is well suited to identify the mechanisms that lead to protective immunity induced by vaccines. We have previously used this platform to describe the very early induction (3 to 7 days) of a network of transcription factors that precede the development of highly integrated innate and adaptive immune responses induced by the Yellow Fever vaccine. In the Systems Biology Core E of this consortium, we aim to (i) provide a centralized sample, reporting, and data management and distribution framework, (ii) perform a comprehensive systems biology assessment of SIV vector-mediated innate and adaptive immune responses, (iii) provide rigorous biostatistical processing and power analysis of microarray and quantitative RT-PCR (QPCR) analysis, and (iv) provide projects and core-integrated immune correlate analysis for SIV vector immune characterization, selection, and study.
The aims of the core will be met via our commercial grade Laboratory Information Management System (LIMS), our SOP-driven, high-throughput. Immune Array platform for microarray analysis, our standardized, semiautomated bioinformatic reporting, and our template-driven data mapping of biological contexts/outcomes to integrate multiple projects and multiple OMIC platforms. We will use these leading edge technologies and bioinformatic strategies to assess the transcriptomic signatures that define early SIV-mediated host responses following different routes of mucosal challenge and the impact of SIV vector-mediated protection.
The impetus to provide an effective HIV vaccine has engaged multiple investigators across the globe and has largely been unsuccessful to date. This program project attempts to utilize novel vaccination strategies and novel technological approaches to develop vaccines in non-human primate models. The Systems Biology Core will use leading technologies in these proposed studies to develop novel vaccines that will eventually be tested in clinical trials in humans.
|Zeng, Ming; Smith, Anthony J; Shang, Liang et al. (2016) Mucosal Humoral Immune Response to SIVmac239âˆ†nef Vaccination and Vaginal Challenge. J Immunol 196:2809-18|
|Barouch, Dan H; Ghneim, Khader; Bosche, William J et al. (2016) Rapid Inflammasome Activation following Mucosal SIV Infection of Rhesus Monkeys. Cell 165:656-67|
|Tartaglia, Lawrence J; Chang, Hui-Wen; Lee, Benjamin C et al. (2016) Production of Mucosally Transmissible SHIV Challenge Stocks from HIV-1 Circulating Recombinant Form 01_AE env Sequences. PLoS Pathog 12:e1005431|
|Martinez-Navio, JosÃ© M; Fuchs, Sebastian P; PedreÃ±o-LÃ³pez, SÃ²nia et al. (2016) Host Anti-antibody Responses Following Adeno-associated Virus-mediated Delivery of Antibodies Against HIV and SIV in Rhesus Monkeys. Mol Ther 24:76-86|
|Larocca, Rafael A; Abbink, Peter; Peron, Jean Pierre S et al. (2016) Vaccine protection against Zika virus from Brazil. Nature 536:474-8|
|Stephenson, Kathryn E; Neubauer, George H; Bricault, Christine A et al. (2016) Antibody Responses After Analytic Treatment Interruption in Human Immunodeficiency Virus-1-Infected Individuals on Early Initiated Antiretroviral Therapy. Open Forum Infect Dis 3:ofw100|
|Stephenson, Kathryn E; D'Couto, Helen T; Barouch, Dan H (2016) New concepts in HIV-1 vaccine development. Curr Opin Immunol 41:39-46|
|Billingsley, James M; Rajakumar, Premeela A; Connole, Michelle A et al. (2015) Characterization of CD8+ T cell differentiation following SIVÎ”nef vaccination by transcription factor expression profiling. PLoS Pathog 11:e1004740|
|Adnan, Sama; Colantonio, Arnaud D; Yu, Yi et al. (2015) CD8 T cell response maturation defined by anentropic specificity and repertoire depth correlates with SIVÎ”nef-induced protection. PLoS Pathog 11:e1004633|
|Barouch, Dan H; Alter, Galit; Broge, Thomas et al. (2015) Protective efficacy of adenovirus/protein vaccines against SIV challenges in rhesus monkeys. Science 349:320-4|
Showing the most recent 10 out of 45 publications