Respiratory viral infections, particularly by human rhinoviruses (HRV), play important roles in inception, development, exacerbation and chronicity of asthma. This program is focused on defining interactions between factors regulating HRV replication and host immune response to viral infection that affect the severity of associated respiratory illness. Each of the projects will use viral diagnostics, and Projects II and 111 require specialized preparations of purified viruses and molecular reagents related to viral biology. The Virology Core is established to provide viral diagnostics and HRV molecular reagents to each of the three proposed projects. Project I will be supported with a repertoire of sensitive and accurate molecular detection assays to identify all common respiratory viruses and the individual species/type/strain of HRV. The viral loads will be quantified by qPCR in a subset of HRV-positive nasal samples from human subjects of Project I for subsequent virus isolation, complete genome sequencing, cDNA cloning and use in Projects ll-lll. In addition, this core will support the in vitro experiments of Projects l-lll by supplying (1) high-titer and uniform HRV virus stocks, including purified viruses (native, radiolabeled and inactivated) of selected HRV serotypes and strains, infected cell lysates of 100 classical HRV-A and HRV-B serotypes and newly identified HRV-C strains, (2) various anti-HRV antibodies, (3) cDNA clones (infectious, chimeric, reporter, replicons) of selected HRV-A, HRV-B and HRV-C types/strains, (4) performing infectivity assays (plaque and TCID50), and (5) culture of airway epithelial cells

Public Health Relevance

Viral respiratory infections with associated airway obstruction and wheezing are the most frequent cause of hospitalization in infants, and in children and adults with asthma. Defining interactions between factors regulating HRV replication and host immune response to viral infection that affect the severity of associated respiratory illness could lead to new strategies to reduce virus-induced wheezing illnesses, and decrease acute exacerbations in people with asthma.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1)
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University of Wisconsin Madison
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Voigt, Emily A; Swick, Adam; Yin, John (2016) Rapid induction and persistence of paracrine-induced cellular antiviral states arrest viral infection spread in A549 cells. Virology 496:59-66
Palmenberg, Ann C (2016) The Language of Life. Annu Rev Virol 3:1-28
Akpinar, Fulya; Timm, Andrea; Yin, John (2016) High-Throughput Single-Cell Kinetics of Virus Infections in the Presence of Defective Interfering Particles. J Virol 90:1599-612
Liu, Yue; Hill, Marchel G; Klose, Thomas et al. (2016) Atomic structure of a rhinovirus C, a virus species linked to severe childhood asthma. Proc Natl Acad Sci U S A 113:8997-9002
Turunen, Riitta; Jartti, Tuomas; Bochkov, Yury A et al. (2016) Rhinovirus species and clinical characteristics in the first wheezing episode in children. J Med Virol 88:2059-2068
Lindsay, Stephen M; Yin, John (2016) Temperature gradients drive radial fluid flow in Petri dishes and multiwell plates. AIChE J 62:2227-2233
Bochkov, Yury A; Watters, Kelly; Basnet, Sarmila et al. (2016) Mutations in VP1 and 3A proteins improve binding and replication of rhinovirus C15 in HeLa-E8 cells. Virology 499:350-360
Fawkner-Corbett, David W; Khoo, Siew Kim; Duarte, Carminha M et al. (2016) Rhinovirus-C detection in children presenting with acute respiratory infection to hospital in Brazil. J Med Virol 88:58-63
Bochkov, Yury A; Gern, James E (2016) Rhinoviruses and Their Receptors: Implications for Allergic Disease. Curr Allergy Asthma Rep 16:30
Warrick, Jay W; Timm, Andrea; Swick, Adam et al. (2016) Tools for Single-Cell Kinetic Analysis of Virus-Host Interactions. PLoS One 11:e0145081

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