The objective of this Core is to provide sequencing services to support the other projects in this Program Project in their efforts to determine the functions of proteins in the M. tuberculosis (Mtb) genome. We will take advantage of next-generation sequencing technology (aka

Public Health Relevance

M. tuberculosis the most prevalent human pathogen worldwide, and a better understanding of the biology of the organism through the functions of genes in the genome is needed for development of new therapies. The goal of this Core is to exploit next-generation DNA sequencing in several ways to provide insight on the functions of genes in the Mtb genome whose functions are currently unknown.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AI107774-01
Application #
8597708
Study Section
Special Emphasis Panel (ZAI1-FDS-M (M1))
Project Start
2013-07-02
Project End
2018-06-30
Budget Start
2013-07-02
Budget End
2014-06-30
Support Year
1
Fiscal Year
2013
Total Cost
$174,795
Indirect Cost
$24,136
Name
Harvard University
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02115
Kery, Mary Beth; Feldman, Monica; Livny, Jonathan et al. (2014) TargetRNA2: identifying targets of small regulatory RNAs in bacteria. Nucleic Acids Res 42:W124-9
DeJesus, Michael A; Ioerger, Thomas R (2013) A Hidden Markov Model for identifying essential and growth-defect regions in bacterial genomes from transposon insertion sequencing data. BMC Bioinformatics 14:303