Core C will store the libraries, and any individual constructs built for this consortium, as bacterial glycerol stocks. Furthermore, the goal of our consortium is to make this technology as """"""""open source"""""""" as possible, and therefore this Core will handle all external requests for shRNAmir libraries or individual constructs. LIAI has an RNAi Center with an excellent infrastructure already in place for large scale library handling, storing, and disseminating. Core C has 3 roles: 1. Storage of the thousands of shRNAmir constructs. 2. Dissemination of libraries through the U19 groups, as bacterial clone replicates. 3. Dissemination of libraries and constructs throughout the NIH IMVC.

Public Health Relevance

When library construction is completed by Core B, with validated sequencing, bacterial colonies will be provided to Core C for (1) re-arraying, (2) Replica plating, (3) cataloguing the constructs in a database connected to the LIAI iLab server, and (4) storage at -80 C in a dedicated freezer.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AI109976-01
Application #
8711629
Study Section
Special Emphasis Panel (ZAI1-ZL-I (J1))
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
1
Fiscal Year
2014
Total Cost
$37,749
Indirect Cost
$6,090
Name
La Jolla Institute
Department
Type
DUNS #
603880287
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Shaw, Laura A; Bélanger, Simon; Omilusik, Kyla D et al. (2016) Id2 reinforces TH1 differentiation and inhibits E2A to repress TFH differentiation. Nat Immunol 17:834-43
Martinez, Gustavo J; Hu, Joyce K; Pereira, Renata M et al. (2016) Cutting Edge: NFAT Transcription Factors Promote the Generation of Follicular Helper T Cells in Response to Acute Viral Infection. J Immunol 196:2015-9
Pedros, Christophe; Zhang, Yaoyang; Hu, Joyce K et al. (2016) A TRAF-like motif of the inducible costimulator ICOS controls development of germinal center TFH cells via the kinase TBK1. Nat Immunol 17:825-33
Crotty, Shane (2015) A brief history of T cell help to B cells. Nat Rev Immunol 15:185-9
Nance, J Philip; Bélanger, Simon; Johnston, Robert J et al. (2015) Bcl6 middle domain repressor function is required for T follicular helper cell differentiation and utilizes the corepressor MTA3. Proc Natl Acad Sci U S A 112:13324-9
Martinez, Gustavo J; Pereira, Renata M; Äijö, Tarmo et al. (2015) The transcription factor NFAT promotes exhaustion of activated CD8⁺ T cells. Immunity 42:265-78
Hatzi, Katerina; Nance, J Philip; Kroenke, Mark A et al. (2015) BCL6 orchestrates Tfh cell differentiation via multiple distinct mechanisms. J Exp Med 212:539-53
Choi, Youn Soo; Gullicksrud, Jodi A; Xing, Shaojun et al. (2015) LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6. Nat Immunol 16:980-90
Nance, J Philip; Bélanger, Simon; Johnston, Robert J et al. (2015) Cutting edge: T follicular helper cell differentiation is defective in the absence of Bcl6 BTB repressor domain function. J Immunol 194:5599-603
Crotty, Shane; Pipkin, Matthew E (2015) In vivo RNAi screens: concepts and applications. Trends Immunol 36:315-22

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