Core C will store the libraries, and any individual constructs built for this consortium, as bacterial glycerol stocks. Furthermore, the goal of our consortium is to make this technology as "open source" as possible, and therefore this Core will handle all external requests for shRNAmir libraries or individual constructs. LIAI has an RNAi Center with an excellent infrastructure already in place for large scale library handling, storing, and disseminating. Core C has 3 roles: 1. Storage of the thousands of shRNAmir constructs. 2. Dissemination of libraries through the U19 groups, as bacterial clone replicates. 3. Dissemination of libraries and constructs throughout the NIH IMVC.
When library construction is completed by Core B, with validated sequencing, bacterial colonies will be provided to Core C for (1) re-arraying, (2) Replica plating, (3) cataloguing the constructs in a database connected to the LIAI iLab server, and (4) storage at -80 C in a dedicated freezer.
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|Crotty, Shane (2014) T follicular helper cell differentiation, function, and roles in disease. Immunity 41:529-42|