Emerging antibiotic resistance is making treatment of infections caused by Acinetobacter baumannii, Klebsiella pneumoniae and other Enterobacteriaceae increasingly ineffective. Many resistance genes are carried on mobile genetic elements and can readily be exchanged between strains and species. This project will use comprehensive genome analysis to improve our understanding of the genetic context of resistance genes, their chromosomal background, and lateral gene transfer. Properties of the drug resistance element transmission process in populations will be analyzed using historical and ongoing collections of isolates from several hospitals. Additional studies are designed to explore the role of mixed infections in genetic exchange and the relationship of pathogens to commensal bacteria in the microbiome. The scope and extent of DNA methylation in A. baumannii and K. pneumoniae will be analyzed using single molecule real time sequencing and the effect on gene expression will be determined. The results should be of value in development of molecular assays to facilitate diagnosis and selection of appropriate therapies for clinical infections.
The emergence of antibiotic resistant bacteria represents a growing healthcare threat. Better understanding of the genes that cause resistance and how they are exchanged among bacteria will help in tracking the organisms that cause infection and in selecting appropriate treatment for patients.
|Shao, Lulu; Fischer, David D; Kandasamy, Sukumar et al. (2016) Comparative In Vitro and In Vivo Studies of Porcine Rotavirus G9P and Human Rotavirus Wa G1P. J Virol 90:142-51|
|Rojas, Laura J; Wright, Meredith S; De La Cadena, Elsa et al. (2016) Initial Assessment of the Molecular Epidemiology of blaNDM-1 in Colombia. Antimicrob Agents Chemother 60:4346-50|
|Chavda, Kalyan D; Chen, Liang; Fouts, Derrick E et al. (2016) Comprehensive Genome Analysis of Carbapenemase-Producing Enterobacter spp.: New Insights into Phylogeny, Population Structure, and Resistance Mechanisms. MBio 7:|
|Rosas-Salazar, Christian; Shilts, Meghan H; Tovchigrechko, Andrey et al. (2016) Differences in the Nasopharyngeal Microbiome During Acute Respiratory Tract Infection With Human Rhinovirus and Respiratory Syncytial Virus in Infancy. J Infect Dis 214:1924-1928|
|Nelson, Martha I; Stucker, Karla M; Schobel, Seth A et al. (2016) Introduction, Evolution, and Dissemination of Influenza A Viruses in Exhibition Swine in the United States during 2009 to 2013. J Virol 90:10963-10971|
|Schobel, Seth A; Stucker, Karla M; Moore, Martin L et al. (2016) Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene. Sci Rep 6:26311|
|Shilts, Meghan H; Rosas-Salazar, Christian; Tovchigrechko, Andrey et al. (2016) Minimally Invasive Sampling Method Identifies Differences in Taxonomic Richness of Nasal Microbiomes in Young Infants Associated with Mode of Delivery. Microb Ecol 71:233-42|
|Chen, Rubing; Puri, Vinita; Fedorova, Nadia et al. (2016) Comprehensive Genome Scale Phylogenetic Study Provides New Insights on the Global Expansion of Chikungunya Virus. J Virol 90:10600-10611|
|Rosas-Salazar, Christian; Shilts, Meghan H; Tovchigrechko, Andrey et al. (2016) Nasopharyngeal Microbiome in Respiratory Syncytial Virus Resembles Profile Associated with Increased Childhood Asthma Risk. Am J Respir Crit Care Med 193:1180-3|
|Nelson, Martha I; Wentworth, David E; Das, Suman R et al. (2016) Evolutionary Dynamics of Influenza A Viruses in US Exhibition Swine. J Infect Dis 213:173-82|
Showing the most recent 10 out of 20 publications