for Overview The proposed Center will perform mechanistic studies of allergen-specific T cell responses in peanut allergic subjects through two synergistic projects. The first project will investigate the phenotypic and functional characteristics of allergen specific T cells in subjects with peanut allergy and subjects that are tolerant to peanut. The second project will investigate changes in the frequency, phenotype, and function of allergen- specific T cells that are associated with immunotherapy. This project will utilize longitudinal clinical samples from a peanut oral immunotherapy trial and samples from a peanut peptide immunotherapy in which subjects will receive intradermal injection of the peanut peptide vaccine. Both projects will utilize cutting edge technologies including metal tag class II tetramer reagents with CyTOF and single cell RNA-seq to study peanut allergen specific T cells. The infrastructure of our Center includes two cores. Core A, the Administrative Core, will oversee scientific progress of the two projects, facilitate data integration between the projects, and provide managerial responsibility and fiscal management for the Center. Core B, the Gene Expression and Systems Immunology Core will be responsible for data analysis for RNA-seq, Flow Cytometry and Mass Cytometry, and will also work with Core A in data management. Benaroya Research Institute has state-of-the- art facilities and technical equipment, established infrastructure for technical support and subject recruitment, and collegial environments to support the research as propose.

Public Health Relevance

for Overview The overall aim of this center is to understand the role of peanut epitope-specific T-cell responses in the pathogenesis and treatment of peanut allergy by utilizing peanut T cell epitope-specific tetramer reagents and other novel assays. New knowledge gained in this proposal should facilitate the practice of precision medicine and the development of novel intervention in treating subjects with peanut allergy.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AI135817-01
Application #
9446095
Study Section
Special Emphasis Panel (ZAI1)
Program Officer
Dong, Gang
Project Start
2018-01-01
Project End
2022-12-31
Budget Start
2018-01-01
Budget End
2018-12-31
Support Year
1
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Benaroya Research Institute at Virginia Mason
Department
Type
DUNS #
076647908
City
Seattle
State
WA
Country
United States
Zip Code
98101