Core Leader:
Ch aim Putterman. A central element of the U19 grant is the collection and characterization of patients with systemic lupus erythematosus (SLE), related autoimmune conditions, and normal controls. This requires protocol-driven identification of subjects, standardized recording of medical history, epidemiological information, family history, and the collection and storage of high-quality biospecimens. Underpinning all of this is a secure, accessible database assuring data integrity and interoperability.
The specific aims of the Clinical Resource Core (CRC) are: 1. Recruitment of subjects with SLE and related conditions, disease controls, and healthy controls; 2. Collection of demographic, clinical, and epidemiological data with storage in a secure, confidential database; 3. Collection and storage of serum, urine, DNA, and RNA for distribution to researchers in this program, from affected and unaffected family members. 4. Immortalization of B cells to provide a resource of cells for isolation of stress-related RNPs and testing of immunomodulatory effects

Public Health Relevance

Several aims in collaborative projects rely on the use of material derived from human subjects. In order to facilitate these projects, we will use a CRC facility for the recruitment subjects, centralized collection of information and specimens for use by multiple investigators, and computational support. Archiving of DNA form affected and unaffected family members will facilitate future genomic sequencing studies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program--Cooperative Agreements (U19)
Project #
4U19CA179564-04
Application #
9124830
Study Section
Special Emphasis Panel (ZRG1-OBT-S)
Project Start
Project End
Budget Start
2016-09-01
Budget End
2017-08-31
Support Year
4
Fiscal Year
2016
Total Cost
$378,584
Indirect Cost
$74,556
Name
Rockefeller University
Department
Type
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Max, Klaas E A; Bertram, Karl; Akat, Kemal Marc et al. (2018) Human plasma and serum extracellular small RNA reference profiles and their clinical utility. Proc Natl Acad Sci U S A 115:E5334-E5343
Suryawanshi, Hemant; Morozov, Pavel; Straus, Alexander et al. (2018) A single-cell survey of the human first-trimester placenta and decidua. Sci Adv 4:eaau4788
Wei, Shan; Weiss, Zachary R; Williams, Zev (2018) Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform. G3 (Bethesda) 8:1649-1657
Gerstberger, Stefanie; Meyer, Cindy; Benjamin-Hong, Sigi et al. (2017) The Conserved RNA Exonuclease Rexo5 Is Required for 3' End Maturation of 28S rRNA, 5S rRNA, and snoRNAs. Cell Rep 21:758-772
Smith, Eve Mary Dorothy; Jorgensen, Andrea Lyn; Midgley, Angela et al. (2017) International validation of a urinary biomarker panel for identification of active lupus nephritis in children. Pediatr Nephrol 32:283-295
Gogakos, Tasos; Brown, Miguel; Garzia, Aitor et al. (2017) Characterizing Expression and Processing of Precursor and Mature Human tRNAs by Hydro-tRNAseq and PAR-CLIP. Cell Rep 20:1463-1475
Zheng, Luqian; Mairhofer, Elisabeth; Teplova, Marianna et al. (2017) Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme. Nat Commun 8:1180
Gao, Pu; Yang, Hui; Rajashankar, Kanagalaghatta R et al. (2016) Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition. Cell Res 26:901-13
Wei, Shan; Williams, Zev (2016) Rapid Short-Read Sequencing and Aneuploidy Detection Using MinION Nanopore Technology. Genetics 202:37-44
Ren, Aiming; VuĊĦurovi?, Nikola; Gebetsberger, Jennifer et al. (2016) Pistol ribozyme adopts a pseudoknot fold facilitating site-specific in-line cleavage. Nat Chem Biol 12:702-8

Showing the most recent 10 out of 23 publications