The main objective of Project 2 is to identify and characterize cortical neurons from layer V and subtypes of astrocytes derived from human induced pluripotent stem cells (iPSCs) and measure their contribution to Autism Spectrum Disorders (ASD). Pyramidal neurons from cortical layer V are linked to primate social behavior and human-related conditions, such as ASD and Williams syndrome (WS). Glia cells have historically played a secondary role in neuroscience but recent data indicating a major contribution of astrocytes to several human neurological disorders has challenged this view. Thus, we propose to apply the design of experiments (DOE), a powerful statistical tool, to improve the differentiation protocols for cortical neurons layer V and astrocytes derived from iPSCs. These will be applied to engineered lines from Project 1 for molecular and cellular assays in the Projects 3, 4 and 5.
| Mesci, Pinar; Macia, Angela; LaRock, Christopher N et al. (2018) Modeling neuro-immune interactions during Zika virus infection. Hum Mol Genet 27:41-52 |
| Chailangkarn, Thanathom; Noree, Chalongrat; Muotri, Alysson R (2018) The contribution of GTF2I haploinsufficiency to Williams syndrome. Mol Cell Probes 40:45-51 |
| Brito, Anita; Russo, Fabiele Baldino; Muotri, Alysson Renato et al. (2018) Autism spectrum disorders and disease modeling using stem cells. Cell Tissue Res 371:153-160 |
| Fernandes, Isabella R; Cruz, Ana C P; Ferrasa, Adriano et al. (2018) Genetic variations on SETD5 underlying autistic conditions. Dev Neurobiol 78:500-518 |
| Mesci, Pinar; Macia, Angela; Moore, Spencer M et al. (2018) Blocking Zika virus vertical transmission. Sci Rep 8:1218 |
| Herai, Roberto H; Negraes, Priscilla D; Muotri, Alysson R (2017) Evidence of nuclei-encoded spliceosome mediating splicing of mitochondrial RNA. Hum Mol Genet 26:2472-2479 |
| Beltrão-Braga, Patricia C B; Muotri, Alysson R (2017) Modeling autism spectrum disorders with human neurons. Brain Res 1656:49-54 |
| Song, Yan; Botvinnik, Olga B; Lovci, Michael T et al. (2017) Single-Cell Alternative Splicing Analysis with Expedition Reveals Splicing Dynamics during Neuron Differentiation. Mol Cell 67:148-161.e5 |
| de Souza, Janaina S; Carromeu, Cassiano; Torres, Laila B et al. (2017) IGF1 neuronal response in the absence of MECP2 is dependent on TRalpha 3. Hum Mol Genet 26:270-281 |
| Shiryaev, Sergey A; Mesci, Pinar; Pinto, Antonella et al. (2017) Repurposing of the anti-malaria drug chloroquine for Zika Virus treatment and prophylaxis. Sci Rep 7:15771 |
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