This proposal, from a group of established scientists at the University of Cincinnati, is in response to RFA DK-10-006 to continue funding for one of six NIH-funded Mouse Metabolic Phenotyping Centers with the mission of providing phenotyping services related to diabetes and its complications to diverse scientists and investigators. Five Cores are proposed: 1) Administrative/Statistical Core will be responsible for processing requests, uploading test results to the national database, perform statistical analyses, take care of budgetary issues, purchase of supplies and equipment, post information about the types of tests offered and protocol used on the Center's web site;2) Animal Care Core will receive, house, feed, and genotype the incoming mice as well as maintain them in excellent condition during the period of phenotypic testing;3) Lipid, Lipoprotein, and Glucose Metabolism Core will study lipid and glucose metabolism and measure gastrointestinal hormones known to influence insulin secretion;4) Cardiovascular Core will provide numerous assessments of cardiovascular complications and tissue inflammation;and 5) Energy Metabolism Core will assess simple and detailed parameters of energy intake (food intake) and energy expenditure (including use of indirect calorimetry) and determine body composition and fat content. We believe that our collective experience and expertise as well as our experience running and operating one of the six centers since July 1, 2001 makes our proposal unique and competitive. We are excited at the prospect of collaborating on this important initiative.
Diabetes is a major health problem, and investigation of many aspects of its physiology and pathophysiology are ongoing. Numerous transgenic and knockout mouse models are being generated for the investigation of both Types I &II diabetes and their co-morbidities. MMPCs will provide detailed metabolic phenotyping of these mouse models and will be potentially useful for understanding diabetes and its complications.
|Harley, Isaac T W; Stankiewicz, Traci E; Giles, Daniel A et al. (2014) IL-17 signaling accelerates the progression of nonalcoholic fatty liver disease in mice. Hepatology 59:1830-9|
|Wang, Fei; Kohan, Alison B; Dong, H Henry et al. (2014) Overexpression of apolipoprotein C-III decreases secretion of dietary triglyceride into lymph. Physiol Rep 2:e00247|
|Poole, Daniel P; Lee, Mike; Tso, Patrick et al. (2014) Feeding-dependent activation of enteric cells and sensory neurons by lymphatic fluid: evidence for a neurolymphocrine system. Am J Physiol Gastrointest Liver Physiol 306:G686-98|
|Tong, Jenny; Davis, Harold W; Summer, Suzanne et al. (2014) Acute administration of unacylated ghrelin has no effect on Basal or stimulated insulin secretion in healthy humans. Diabetes 63:2309-19|
|Li, Jibiao; Bi, Lipeng; Hulke, Michelle et al. (2014) Fish oil and fenofibrate prevented phosphorylation-dependent hepatic sortilin 1 degradation in Western diet-fed mice. J Biol Chem 289:22437-49|
|Li, Xiaoming; Xu, Min; Wang, Fei et al. (2014) Apolipoprotein A-IV reduces hepatic gluconeogenesis through nuclear receptor NR1D1. J Biol Chem 289:2396-404|
|Able, Jessica A; Liu, Yanhong; Jandacek, Ronald et al. (2014) Omega-3 fatty acid deficient male rats exhibit abnormal behavioral activation in the forced swim test following chronic fluoxetine treatment: association with altered 5-HT1A and alpha2A adrenergic receptor expression. J Psychiatr Res 50:42-50|
|Lo, Chunmin C; Davidson, W Sean; Hibbard, Stephanie K et al. (2014) Intraperitoneal CCK and fourth-intraventricular Apo AIV require both peripheral and NTS CCK1R to reduce food intake in male rats. Endocrinology 155:1700-7|
|McNamara, Robert K; Strimpfel, Jennifer; Jandacek, Ronald et al. (2014) Detection and Treatment of Long-Chain Omega-3 Fatty Acid Deficiency in Adolescents with SSRI-Resistant Major Depressive Disorder. PharmaNutrition 2:38-46|
|Nohara, Kazunari; Laque, Amanda; Allard, Camille et al. (2014) Central mechanisms of adiposity in adult female mice with androgen excess. Obesity (Silver Spring) 22:1477-84|
Showing the most recent 10 out of 113 publications