The Yale Mouse Metabolic Phenotyping Center (MMPC) brings together a multidisciplinary group of investigators at Yale to develop and apply novel in vivo and in vitro techniques to assess metabolic alterations in transgenic mice. The cornerstone of the Center is its Research Cores that will provide NIH funded scientists access to unique resources and standardized methods to characterize their transgenic mice. The Yale MMPC will consists of six cores; 1) the Administrative Core 2) the Animal Care Core 3) the In Vivo Metabolism Core, 4) the In Vitro Metabolism Core, 5) the Magnetic Resonance Spectroscopy (MRS)/Magnetic Resonance Imaging (MRI) Core, and 6) the Analytical Core. The Administrative Core will oversee the operation of the Center as well as the Pilot and Feasibility Project Program. The Animal Core will provide a centralized facility for coordinating receiving, screening and monitoring mice from investigators. The In Vivo Metabolism Core will perform glucose-insulin clamps using both radioactive and stable isotope technology to assess insulin action and energy metabolism in liver and skeletal muscle of awake mice. The MRS/MRI Core will perform noninvasive measurements of intracellular metabolites in muscle, liver and brain of transgenic mice using 13C/31P/1H MR spectroscopy as well as whole body measurement of fat and muscle tissue with MRI. The In Vitro Metabolism Core will perform phenotyping tests on isolated perfused organs (heart, liver, skeletal muscle, pancreatic beta cells) using both stable and radioactive tracer methodology in conjunction with GC/MS and NMR spectroscopic analysis. The Analytical Core will provide GC-MS and LC/MS/MS analysis of plasma and tissue samples obtained from protocols performed in the In Vivo, MRS/MRI and the In Vitro Metabolic Cores. Overall it is anticipated that the Yale MMPC will be a national research center that provides NIH funded investigators both inside and outside Yale access to unique, state-of-the-art, standardized methods to further characterize their novel transgenic and knockout mouse models of complex metabolic diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
3U24DK059635-04S1
Application #
6944118
Study Section
Special Emphasis Panel (ZDK1)
Program Officer
Abraham, Kristin M
Project Start
2001-07-01
Project End
2006-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
4
Fiscal Year
2004
Total Cost
$32,700
Indirect Cost
Name
Yale University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Majtan, Tomas; Jones Jr, Wendell; Krijt, Jakub et al. (2018) Enzyme Replacement Therapy Ameliorates Multiple Symptoms of Murine Homocystinuria. Mol Ther 26:834-844
Perry, Rachel J; Wang, Yongliang; Cline, Gary W et al. (2018) Leptin Mediates a Glucose-Fatty Acid Cycle to Maintain Glucose Homeostasis in Starvation. Cell 172:234-248.e17
Lawan, Ahmed; Min, Kisuk; Zhang, Lei et al. (2018) Skeletal Muscle-Specific Deletion of MKP-1 Reveals a p38 MAPK/JNK/Akt Signaling Node That Regulates Obesity-Induced Insulin Resistance. Diabetes 67:624-635
Vatner, Daniel F; Goedeke, Leigh; Camporez, Joao-Paulo G et al. (2018) Angptl8 antisense oligonucleotide improves adipose lipid metabolism and prevents diet-induced NAFLD and hepatic insulin resistance in rodents. Diabetologia 61:1435-1446
Wang, Yongliang; Nasiri, Ali R; Damsky, William E et al. (2018) Uncoupling Hepatic Oxidative Phosphorylation Reduces Tumor Growth in Two Murine Models of Colon Cancer. Cell Rep 24:47-55
Qiu, Yang; Perry, Rachel J; Camporez, João-Paulo G et al. (2018) In vivo studies on the mechanism of methylene cyclopropyl acetic acid and methylene cyclopropyl glycine-induced hypoglycemia. Biochem J 475:1063-1074
Budatha, Madhusudhan; Zhang, Jiasheng; Zhuang, Zhen W et al. (2018) Inhibiting Integrin ?5 Cytoplasmic Domain Signaling Reduces Atherosclerosis and Promotes Arteriogenesis. J Am Heart Assoc 7:
Jelenik, Tomas; Flögel, Ulrich; Álvarez-Hernández, Elisa et al. (2018) Insulin Resistance and Vulnerability to Cardiac Ischemia. Diabetes 67:2695-2702
Corbit, Kevin C; Camporez, João Paulo G; Edmunds, Lia R et al. (2018) Adipocyte JAK2 Regulates Hepatic Insulin Sensitivity Independently of Body Composition, Liver Lipid Content, and Hepatic Insulin Signaling. Diabetes 67:208-221
Perry, Rachel J; Peng, Liang; Cline, Gary W et al. (2018) Mechanisms by which a Very-Low-Calorie Diet Reverses Hyperglycemia in a Rat Model of Type 2 Diabetes. Cell Metab 27:210-217.e3

Showing the most recent 10 out of 255 publications