The Metabolomics Core provides a facility to determine the phenotype of metabolite and hormone profiles of transgenic mouse models of diabetes and its complications. The services provided by the Metabolomics Core include the extraction, purification, derivatization, and instrumental analysis of metabolites, hormones, and selected enzyme activities from tissues and plasma. The methods have been scaled to account for the minimal amounts of tissues (-10 to 25 mg, and plasma volumes of - 5 to 25 pi) that can be provided by investigators.. The facility is equipped with an array of state-of-the-art instrumentation, including GC-MS, LC/MS/MS, HPLC, and NMR, providing the flexibility and resources for analyses of metabolite concentrations as well as sotopic enrichments. Metabolic panels of serum, plasma, and urine, including Chem 7, liver function tests lipid profiles, and divalent cations, are obtained using the COBAS MIRA system. We have optimized the COBAS Mira analytical system for analysis of mouse samples such that required volumes average -20 pi of serum for any combination of 4 tests. These plasma chemistry assays fill a need with very high demand for mouse studies, as reflected in our work flow over the last funding period. GC/MS and LC/MS/MS analyses provide complete profiles of chain length distribution of fatty acid metabolites, included free fatty acids, fatty acyl-CoA esters, diacylglycerols, ceramides. Other LC/MS/MS that are offered and are in high-demand include intermediates of glucose metabolism for metabolic flux measurements, and creatinines for renal clearance. The Metabolomics Core is a resource lab for the analysis of samples generated during the course of experiments of the Integrative Physiology Core at the Yale MMPC, and also provides the same array of assays to other outside investigators to assist in phenotyping of transgenic mouse models of diabetes Samples are analyzed on a fee-for-service basis with the charge per assay based on policy determined by the National MMPC Executive Committee, designed to provide substantial savings to NIH-funded users compared to commercial vendors. The Core has an active Research and Development Program to develop new analytical protocols to address new and exciting developments in the biochemistry of diabetes, obesity, and associated complications

Public Health Relevance

The Core has an active Research and Development Program to develop new analytical protocols to address new and exciting developments in the biochemistry of diabetes, obesity, and associated complications

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK059635-07
Application #
8381212
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
7
Fiscal Year
2012
Total Cost
$196,424
Indirect Cost
$78,096
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Petersen, Max C; Madiraju, Anila K; Gassaway, Brandon M et al. (2016) Insulin receptor Thr1160 phosphorylation mediates lipid-induced hepatic insulin resistance. J Clin Invest 126:4361-4371
Zaha, Vlad G; Qi, Dake; Su, Kevin N et al. (2016) AMPK is critical for mitochondrial function during reperfusion after myocardial ischemia. J Mol Cell Cardiol 91:104-13
Perry, Rachel J; Petersen, Kitt Falk; Shulman, Gerald I (2016) Pleotropic effects of leptin to reverse insulin resistance and diabetic ketoacidosis. Diabetologia 59:933-7
Li, Yuanyuan; Tian, Xin; Ma, Ming et al. (2016) Deletion of ADP Ribosylation Factor-Like GTPase 13B Leads to Kidney Cysts. J Am Soc Nephrol 27:3628-3638
Kumamoto, Yosuke; Camporez, Joao Paulo G; Jurczak, Michael J et al. (2016) CD301b(+) Mononuclear Phagocytes Maintain Positive Energy Balance through Secretion of Resistin-like Molecule Alpha. Immunity 45:583-96
Costa, Diana K; Huckestein, Brydie R; Edmunds, Lia R et al. (2016) Reduced intestinal lipid absorption and body weight-independent improvements in insulin sensitivity in high-fat diet-fed Park2 knockout mice. Am J Physiol Endocrinol Metab 311:E105-16
Khan, Nayaab S; Song, Chi Young; Thirunavukkarasu, Shyamala et al. (2016) Cytosolic Phospholipase A2α Is Essential for Renal Dysfunction and End-Organ Damage Associated With Angiotensin II-Induced Hypertension. Am J Hypertens 29:258-65
Chen, Pei-Yu; Qin, Lingfeng; Li, Guangxin et al. (2016) Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression. EMBO Mol Med 8:712-28
Perry, Rachel J; Borders, Candace B; Cline, Gary W et al. (2016) Propionate Increases Hepatic Pyruvate Cycling and Anaplerosis and Alters Mitochondrial Metabolism. J Biol Chem 291:12161-70
Pesta, Dominik H; Tsirigotis, Dimitrios N; Befroy, Douglas E et al. (2016) Hypophosphatemia promotes lower rates of muscle ATP synthesis. FASEB J 30:3378-3387

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