Metabolic dysregulation is central to the pathogenesis of diabetes and obesity. The Metabolic Pathophysiology Core (MPC) provides services to comprehensively phenotype metabolic processes in mouse models. The MPC has three Subcores: Metabolic Regulation, Tissue and In Vivo Imaging and Bariatric Surgery. The Metabolic Regulation Subcore provides tools to accurately assess metabolism in healthy, conscious, unstressed mice. This Subcore uses unique skills in chronically implanting catheters into the carotid artery and jugular vein to perform glucose clamps in the mouse. In addition, it has developed techniques to catheterize the portal vein and stomach to deliver hormones and nutrients by their physiologic routes. This Subcore also provides services to assess the components of energy balance (energy expenditure, food intake, activity, body composition). The MPC provides not only standardized tests for metabolic and endocrine assessments, but is also able to adapt using more sophisticated protocols. Additional services include in vitro organ perfusion techniques (liver, pancreas, hindlimb). It partners with VDRTC Islet Procurement and Analysis Core to provide high quality islets to investigators as well as tools to characterize islet function. The Tissue and In Vivo Imaging Subcore offers novel imaging technology to study metabolic processes in real time at the molecular level. Resources ofthis Subcore include: 1) a multi-photon excitation confocal microscope to visualize real time kinetics of calcium, NAD(P)H, and pH and fluorescent probes in cells and in situ whole organ preparations;and 2) bioluminescence imaging, to assay real-time kinetics of gene expression in intact mice by combining luciferase as a reporter gene with a highly sensitive optical single photon detection system. The Bariatric Surgery Subcore provides mice with restrictive or bypass surgical procedures that recapitulate the surgical procedures performed in humans for weight loss. The MPC, by virtue of its novel subcores, provides state-of-the-art technology to delineate the mechanism for the phenotypic expression of metabolic disorders in mice.

Public Health Relevance

This core provides services to investigators to help them quantify the impact of a genetic or pharmacologic manipulation on metabolic and endocrine processes in mice with the hope of helping them discover how to cure metabolic disease such as obesity and diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK059637-12
Application #
8379711
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
12
Fiscal Year
2012
Total Cost
$649,451
Indirect Cost
$233,136
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Williams, Ashley S; Trefts, Elijah; Lantier, Louise et al. (2017) Integrin-Linked Kinase Is Necessary for the Development of Diet-Induced Hepatic Insulin Resistance. Diabetes 66:325-334
Peterson, Kristin R; Flaherty, David K; Hasty, Alyssa H (2017) Obesity Alters B Cell and Macrophage Populations in Brown Adipose Tissue. Obesity (Silver Spring) 25:1881-1884
Woodard, Lauren E; Cheng, Jizhong; Welch, Richard C et al. (2017) Kidney-specific transposon-mediated gene transfer in vivo. Sci Rep 7:44904
Wanders, Desiree; Forney, Laura A; Stone, Kirsten P et al. (2017) FGF21 Mediates the Thermogenic and Insulin-Sensitizing Effects of Dietary Methionine Restriction but Not Its Effects on Hepatic Lipid Metabolism. Diabetes 66:858-867
Cadar, Adrian G; Feaster, Tromondae K; Durbin, Matthew D et al. (2017) Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique. Curr Protoc Stem Cell Biol 42:4A.14.1-4A.14.7
Felsted, Jennifer A; Chien, Cheng-Hao; Wang, Dongqing et al. (2017) Alpha2delta-1 in SF1+ Neurons of the Ventromedial Hypothalamus Is an Essential Regulator of Glucose and Lipid Homeostasis. Cell Rep 21:2737-2747
Hinder, Lucy M; O'Brien, Phillipe D; Hayes, John M et al. (2017) Dietary reversal of neuropathy in a murine model of prediabetes and metabolic syndrome. Dis Model Mech 10:717-725
Jao, Li-En; Akef, Abdalla; Wente, Susan R (2017) A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies. Mol Biol Cell 28:120-127
Dickerson, Matthew T; Vierra, Nicholas C; Milian, Sarah C et al. (2017) Osteopontin activates the diabetes-associated potassium channel TALK-1 in pancreatic ?-cells. PLoS One 12:e0175069
Traver, Geri; Mont, Stacey; Gius, David et al. (2017) Loss of Nrf2 promotes alveolar type 2 cell loss in irradiated, fibrotic lung. Free Radic Biol Med 112:578-586

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