Core C: Analytical &Metabolomic The Case MMPC specializes in the use of stable isotopes, mass spectrometry and metabolomics to investigate the regulation of pathways of intermediary metabolism in vivo and ex vivo. Emphasis is on quantitative measurements of inter-organ and intra-organ carbon fluxes. Our strategy links clinical-type studies (limited to body fluids) and pure biochemical studies (such as the kinetics of purified enzymes). The association of metabolomics and mass isotopomer analysis provides a wealth of information on the regulation of known pathways. It also allows to identify new pathways. Metabolomics developed initially as a non-targeted strategy aimed at gathering sufficient data on a (patho)physiological process (biomarkers), so that one could formulate hypotheses to be tested. A variant of this strategy is to target metabolomic studies to classes of compounds, the concentrations and labeling patterns of which can provide much new information: citric acid cycle intermediates, (hydroxy)acids, acyl-CoA esters, acylcarnitine esters. The Case MMPC occupies a specialized niche in the MMPC consortium, complementing the expertise of the other centers, but with a healthy degree of overlap with the Vanderbiit, Yale, Dallas and Cincinnati centers.
The specific aims of the Analytical &Metabolomic Core are: 1. To help users in the design, implementation, analytical procedures and interpretation of metabolic studies conducted either, (i) entirely in the user's lab, (ii) entirely in the Case MMPC, or (iii) partly in the user's lab (animal experiments) and partly in the MMPC (analyses of tissues and body fluids shipped from the user's lab). 2. To set up, at users'request, tests and analytical procedures not currently listed on the web page of the Case MMPC. 3. To develop analytical techniques and isotopic strategies in anticipation of users'needs. 4. To train US and foreign scientists in the proper use of isotopes for metabolic studies. This training takes to form of (i) mentoring individual users, and (ii) contribution to an annual MMPC course.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK076174-04
Application #
8708044
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
4
Fiscal Year
2014
Total Cost
$407,517
Indirect Cost
$147,952
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Mottillo, Emilio P; Balasubramanian, Priya; Lee, Yun-Hee et al. (2014) Coupling of lipolysis and de novo lipogenesis in brown, beige, and white adipose tissues during chronic ?3-adrenergic receptor activation. J Lipid Res 55:2276-86
Kelly, Karen R; Navaneethan, Sankar D; Solomon, Thomas P J et al. (2014) Lifestyle-induced decrease in fat mass improves adiponectin secretion in obese adults. Med Sci Sports Exerc 46:920-6
Du, Jinwei; Li, Qiang; Tang, Fangqiang et al. (2014) Cited2 is required for the maintenance of glycolytic metabolism in adult hematopoietic stem cells. Stem Cells Dev 23:83-94
Cannon, Matthew V; Buchner, David A; Hester, James et al. (2014) Maternal nutrition induces pervasive gene expression changes but no detectable DNA methylation differences in the liver of adult offspring. PLoS One 9:e90335
Tao, Hanlin; Zhang, Yong; Zeng, Xiangang et al. (2014) Niclosamide ethanolamine-induced mild mitochondrial uncoupling improves diabetic symptoms in mice. Nat Med 20:1263-9
Khan, Shenaz; Abu Jawdeh, Bassam G; Goel, Monu et al. (2014) Lipotoxic disruption of NHE1 interaction with PI(4,5)P2 expedites proximal tubule apoptosis. J Clin Invest 124:1057-68
Sadhukhan, Sushabhan; Han, Yong; Jin, Zhicheng et al. (2014) Glutathionylated 4-hydroxy-2-(E)-alkenal enantiomers in rat organs and their contributions toward the disposal of 4-hydroxy-2-(E)-nonenal in rat liver. Free Radic Biol Med 70:78-85
Ruiz, Rafaela; Jideonwo, Victoria; Ahn, Miwon et al. (2014) Sterol regulatory element-binding protein-1 (SREBP-1) is required to regulate glycogen synthesis and gluconeogenic gene expression in mouse liver. J Biol Chem 289:5510-7
Feng, Xiujing; Scott, Anthony; Wang, Yong et al. (2014) PTPRT regulates high-fat diet-induced obesity and insulin resistance. PLoS One 9:e100783
Prince, Allison; Zhang, Yifan; Croniger, Colleen et al. (2013) Oxidative metabolism: glucose versus ketones. Adv Exp Med Biol 789:323-8

Showing the most recent 10 out of 11 publications