Hormones produced by endocrine organs including adipose tissue and inflammatory cytokines produced by immune cells and adipocytes play a major role in regulating energy balance and glucose metabolism. Tissue lipid and intracellular lipid-defived metabolites also modulate metabolic signaling pathways and affect insulin resistance. To understand the underlying basis of metabolic phenotypes of transgenic mice with altered metabolism, the Analytical Core measures serum and tissue factors known to affect energy balance and glucose homeostasis. The goal ofthe Analytical Core is to provide standardized and high-throughput analytical measurements using state-of-the-art instruments in serum and tissue samples obtained from transgenic mice. The Core operates under the supen/ision of Dr. David Harlan as the Core Director and Drs. Randall Friedline, Klaus Pechhold, and Scott Shaffer as co-Directors. The Analytical Core provides the following services: 1) High-throughput and multiplexed measurement of serum/tissue/cell culture levels of hormones, cytokines, and chemokines using Luminex and Meso Scale Discovery multiplexing technology, 2) High-throughput measurement of blood/tissue/urine levels of metabolites and electrolytes using a Cobas Clinical Chemistry Analyzer, 3) Molecular analysis of intracellular signaling pathways associated with insulin action, glucose metabolism, and inflammation, 4) Measurement of intracellular levels of lipids and lipid-derived metabolites using triple quadrupole mass spectrometry, and 5) Assessment of islet histology and gene transcriptional analysis of pancreatic islet cell subsets. The Analytical Core performs quality and reproducible measurements to support the Metabolism and Cardiovascular Complications Core for comprehensive phenotyping of transgenic mice.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Massachusetts Medical School Worcester
United States
Zip Code
Kim, Mi-Sung; Krawczyk, Sarah A; Doridot, Ludivine et al. (2016) ChREBP regulates fructose-induced glucose production independently of insulin signaling. J Clin Invest 126:4372-4386
Roth Flach, Rachel J; Danai, Laura V; DiStefano, Marina T et al. (2016) Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291:16221-30
Jara, Adam; Liu, Xingbo; Sim, Don et al. (2016) Cardiac-Specific Disruption of GH Receptor Alters Glucose Homeostasis While Maintaining Normal Cardiac Performance in Adult Male Mice. Endocrinology 157:1929-41
Friedline, Randall H; Ko, Hwi Jin; Jung, Dae Young et al. (2016) Genetic ablation of lymphocytes and cytokine signaling in nonobese diabetic mice prevents diet-induced obesity and insulin resistance. FASEB J 30:1328-38
Winnay, Jonathon N; Solheim, Marie H; Dirice, Ercument et al. (2016) PI3-kinase mutation linked to insulin and growth factor resistance in vivo. J Clin Invest 126:1401-12
Vernia, Santiago; Morel, Caroline; Madara, Joseph C et al. (2016) Excitatory transmission onto AgRP neurons is regulated by cJun NH2-terminal kinase 3 in response to metabolic stress. Elife 5:e10031
Min, So Yun; Kady, Jamie; Nam, Minwoo et al. (2016) Human 'brite/beige' adipocytes develop from capillary networks, and their implantation improves metabolic homeostasis in mice. Nat Med 22:312-8
Li, Zhu; Frey, Julie L; Wong, G William et al. (2016) Glucose Transporter-4 Facilitates Insulin-Stimulated Glucose Uptake in Osteoblasts. Endocrinology 157:4094-4103
Ejaz, Asma; Martinez-Guino, Laura; Goldfine, Allison B et al. (2016) Dietary Betaine Supplementation Increases Fgf21 Levels to Improve Glucose Homeostasis and Reduce Hepatic Lipid Accumulation in Mice. Diabetes 65:902-12
Ghanem, Simona S; Heinrich, Garrett; Lester, Sumona G et al. (2016) Increased Glucose-induced Secretion of Glucagon-like Peptide-1 in Mice Lacking the Carcinoembryonic Antigen-related Cell Adhesion Molecule 2 (CEACAM2). J Biol Chem 291:980-8

Showing the most recent 10 out of 39 publications