The Metabolism Core is designed to conduct in vivo and physiological experiments in conscious mice for the purpose of detailed metabolic phenotyping of transgenic mouse models useful for understanding obesity, diabetes and its complications. The Core operates under the supervision of Drs. Jason Kim (Director), Dae Young Jung (co-Director), and Hwi Jin Ko (co-Director) who oversee day-to-day operation of the Core and interact with the users of the UMass MMPC. The principal functions of the Core are to perform the following experiments: 1) hyperinsulinemic-euglycemic clamp to assess organ-specific insulin action and glucose metabolism in conscious mice, 2) hyperglycemic clamp to assess in vivo pancreatic beta-cell function in conscious mice, 3) iv or ip glucose/insulin tolerance tests, 4) survival surgery of jugular vein cannulation for in vivo experiments, 5) implementation of osmotic pumps for chronic delivery of drugs or other agents, 6) application of special diet (e.g.. high-fat diet) to alter energy balance and induce obesity in mice. 7) acute lipid infusion, acute/chronic delivery of cytokines and phloridzin, and STZ injection to alter glucose homeostasis. 8) non-invasive measurement of energy expenditure, respiratory exchange ratio, physical activity, and food/water intake in conscious mice using metabolic cages. 9) non-invasive assessment of whole body, organ, and tissue biopsy composition of fat/lean/water mass in conscious mice using 1 H-MRS. 10) implementation of cage wheel for exercise study, and 12) biochemical analysis for intracellular metabolic flux measurement (glycolysis, glycogen synthesis). The Metabolism Core regulariy interacts with the Analytical Core for complementary, high-throughput analytical assays to measure hormones, cytokines and metabolites using serum samples obtained at the end of metabolic experiments. Also, the Metabolism Core interacts and with the Cardiovascular Complications Core for additional phenotyping associated with cardiovascular functions. The goal of the Metabolism Core is to use an array of elegant, non-invasive and in vivo experiments to obtain a robust set of metabolic data from individual mouse in order to provide mechanistic insights into the role of selected gene on obesity, insulin resistance, and diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK093000-04
Application #
8708056
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
4
Fiscal Year
2014
Total Cost
$246,750
Indirect Cost
$96,750
Name
University of Massachusetts Medical School Worcester
Department
Type
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
Dagdeviren, Sezin; Jung, Dae Young; Friedline, Randall H et al. (2017) IL-10 prevents aging-associated inflammation and insulin resistance in skeletal muscle. FASEB J 31:701-710
Kant, Shashi; Standen, Claire L; Morel, Caroline et al. (2017) A Protein Scaffold Coordinates SRC-Mediated JNK Activation in Response to Metabolic Stress. Cell Rep 20:2775-2783
Virtue, Anthony; Johnson, Candice; Lopez-PastraƱa, Jahaira et al. (2017) MicroRNA-155 Deficiency Leads to Decreased Atherosclerosis, Increased White Adipose Tissue Obesity, and Non-alcoholic Fatty Liver Disease: A NOVEL MOUSE MODEL OF OBESITY PARADOX. J Biol Chem 292:1267-1287
Suk, Sujin; Kwon, Gyoo Taik; Lee, Eunjung et al. (2017) Gingerenone A, a polyphenol present in ginger, suppresses obesity and adipose tissue inflammation in high-fat diet-fed mice. Mol Nutr Food Res 61:
DiStefano, Marina T; Roth Flach, Rachel J; Senol-Cosar, Ozlem et al. (2016) Adipocyte-specific Hypoxia-inducible gene 2 promotes fat deposition and diet-induced insulin resistance. Mol Metab 5:1149-1161
Dagdeviren, Sezin; Jung, Dae Young; Lee, Eunjung et al. (2016) Altered Interleukin-10 Signaling in Skeletal Muscle Regulates Obesity-Mediated Inflammation and Insulin Resistance. Mol Cell Biol 36:2956-2966
Vernia, Santiago; Edwards, Yvonne Jk; Han, Myoung Sook et al. (2016) An alternative splicing program promotes adipose tissue thermogenesis. Elife 5:
Ghanem, Simona S; Heinrich, Garrett; Lester, Sumona G et al. (2016) Increased Glucose-induced Secretion of Glucagon-like Peptide-1 in Mice Lacking the Carcinoembryonic Antigen-related Cell Adhesion Molecule 2 (CEACAM2). J Biol Chem 291:980-8
Friedline, Randall H; Ko, Hwi Jin; Jung, Dae Young et al. (2016) Genetic ablation of lymphocytes and cytokine signaling in nonobese diabetic mice prevents diet-induced obesity and insulin resistance. FASEB J 30:1328-38
Senol-Cosar, Ozlem; Flach, Rachel J Roth; DiStefano, Marina et al. (2016) Tenomodulin promotes human adipocyte differentiation and beneficial visceral adipose tissue expansion. Nat Commun 7:10686

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