The West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC) will offer integrated services through a Metabolomics Central Service Core, and offer advanced services, including method developments, through the Metabolomics Advanced Services Core. Combined, the WC3MRC commands over 30 mass spectrometers, 5 NMR instruments and cutting-edge imaging equipment, and computer clusters with in-house as well as open-access and commercial software for metabolomic data acquisition, data processing, and data analysis including pathway mapping. Statistical support, tool development and advanced metabolic network analysis will be conducted in the Genomics Integration Core, while pilot and feasibility projects as well as courses and workshops will be organized by the Promotion and Outreach Core, to be led by the UC Davis Clinical and Translational Science Center (CISC). Overall management and data transfer to the Data Center will be performed by the Administrative Core. Specifically, the WC3MRC will be the first center in the United States that offers quantitative targeting of over 1,000 identified metabolites over a wide variety of biochemical pathways. For all these metabolites, reference standards are available and sample preparation, mass spectra and chromatographic conditions have been validated. Additionally, the WC3MRC will use untargeted metabolomic methods by accurate mass spectrometry for discovery-driven projects, including compound identifications. Methods used in the Advanced Core laboratories will be robotized and transferred to the Central Service Core for use by recharge fee services. Novel services and tools will be developed, ranging from isotope-labeled flux analysis to image guided metabolomics that will link to the established clientele using the imaging facility. Pathway annotations will be improved through curation of HumanCyc enzymes and by using InChI structure identifiers that will be used to construct complete metabolic networks that are subsequently used for pathway over enrichment statistical analysis. Both local and regional scientists will be engaged through annual, competitive pilot and feasibility awards. Participation and award criteria have been worked out and will be conducted by the CTSC. Training programs will educate the next generation of metabolomics scientists, both on the technical and the medical level.

Public Health Relevance

Better understanding of metabolism is highly relevant for fighting major diseases in the United States, from diabetes to cancer and cardiovascular diseases. The new center for comprehensive metabolic analysis at UC Davis will serve clinical and biomedical researchers across the West Coast with access to cutting-edge tools, collaborations and interpretation of data.

Agency
National Institute of Health (NIH)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK097154-03
Application #
8732629
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Maruvada, Padma
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California Davis
Department
Physiology
Type
Schools of Medicine
DUNS #
City
Davis
State
CA
Country
United States
Zip Code
95618
Barkal, Layla J; Theberge, Ashleigh B; Guo, Chun-Jun et al. (2016) Microbial metabolomics in open microscale platforms. Nat Commun 7:10610
Cajka, Tomas; Fiehn, Oliver (2016) Toward Merging Untargeted and Targeted Methods in Mass Spectrometry-Based Metabolomics and Lipidomics. Anal Chem 88:524-45
Shih, P B; Yang, J; Morisseau, C et al. (2016) Dysregulation of soluble epoxide hydrolase and lipidomic profiles in anorexia nervosa. Mol Psychiatry 21:537-46
Kind, Tobias; Cho, Eunho; Park, Taeeun D et al. (2016) Interstitial Cystitis-Associated Urinary Metabolites Identified by Mass-Spectrometry Based Metabolomics Analysis. Sci Rep 6:39227
Fahrmann, Johannes F; Grapov, Dmitry; DeFelice, Brian C et al. (2016) Serum phosphatidylethanolamine levels distinguish benign from malignant solitary pulmonary nodules and represent a potential diagnostic biomarker for lung cancer. Cancer Biomark 16:609-17
Shahin, Mohamed H; Gong, Yan; McDonough, Caitrin W et al. (2016) A Genetic Response Score for Hydrochlorothiazide Use: Insights From Genomics and Metabolomics Integration. Hypertension 68:621-9
Chen, Zhaohui; Kim, Jayoung (2016) Urinary proteomics and metabolomics studies to monitor bladder health and urological diseases. BMC Urol 16:11
Zhao, Yan; Wan, Debin; Yang, Jun et al. (2016) Catalytic Activities of Tumor-Specific Human Cytochrome P450 CYP2W1 Toward Endogenous Substrates. Drug Metab Dispos 44:771-80
Fiehn, Oliver (2016) Metabolomics by Gas Chromatography-Mass Spectrometry: Combined Targeted and Untargeted Profiling. Curr Protoc Mol Biol 114:30.4.1-30.4.32
Fahrmann, Johannes F; Grapov, Dmitry; Phinney, Brett S et al. (2016) Proteomic profiling of lung adenocarcinoma indicates heightened DNA repair, antioxidant mechanisms and identifies LASP1 as a potential negative predictor of survival. Clin Proteomics 13:31

Showing the most recent 10 out of 105 publications