The study of the the low-molecular weight complement of cells, tissues, and biological fluids can be useful in assessing human health or the biochemical effects of exposures or pharmacological treatments on physiological systems. RTI is able to provide a well-established and uniquely broad range of experience and expertise with LC/MS-based analytical methods that are widely used in metabolomics research. With Dr. Jeremey Nicholson, Dr. Elaine Holmes, and Dr. Ian Wilson serving as co-investigators and consultants to the RCMRC, the proposed LC/MS-based metabolomics technology core will ensure that clients have access to the most recent methods available for data acquaition, analysis, and interpretation. Hence, the specific aims of the proposed LC/MS core are designed to leverage these critical capabilities to meet and exceed the requirements of the RCMRC and the service expectations of its scientific researchers. By integrating these capabilities and expertise with other cores, the LC/MS core at RTI will provide (1) consulting and educational outreach services;(2) the analytical capacity for the conduct of standardized LC/MS-based metabolomic methods and the development and inclusion of new methods and technology to meet the needs of ongoing and planned metabolomics studies, and (3) raw data and summary statistics in a manner consistent with the requirements ofthe DRCC and the needs ofthe client. Our LC/MS broad-spectrum metabolomics approach is designed to look at nonpolar and polar analytes using reverse phase and normal phase (HILIC) chromatographic methods coupled to time-of-flight mass spectrometry. Targeted metabolomic studies are supported on triple quadrupoles or Q-trap platforms with chromatographic conditions optimized for the target group of metabolites, and employ stable-isotope-dilution whenever possible. RTI is committed to providing a sustained infrastructure of staff, facilities, and instrumentation to support these efforts, and the RCMRC intends to continuously integrate the broad-spectrum metabolomics data with metabolite identification and peak matching data to generate a comprehensive reference database, and make it available as a resource to the metabolomics research community.

Public Health Relevance

The LC/MS core will generate and assess metabolomics data using the most current and reliable methods available, evaluate and develop new methodologies, and assist our clients in the design of biological studies, the interpretation of the data, and the integration of metabolomics data with their genomic and proteomic data. In this way the core will work to improve the human condition, which is consistent with RTI's mission.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Research Triangle Institute
Research Triangle
United States
Zip Code
Johnson-Weaver, Brandi T; McRitchie, Susan; Mercier, Kelly A et al. (2017) Effect of endotoxin and alum adjuvant vaccine on peanut allergy. J Allergy Clin Immunol :
McClenathan, Bruce M; Stewart, Delisha A; Spooner, Christina E et al. (2017) Metabolites as biomarkers of adverse reactions following vaccination: A pilot study using nuclear magnetic resonance metabolomics. Vaccine 35:1238-1245
Chou, Hsuan; Pathmasiri, Wimal; Deese-Spruill, Jocelin et al. (2017) Metabolomics reveal physiological changes in mayfly larvae (Neocloeon triangulifer) at ecological upper thermal limits. J Insect Physiol 101:107-112
Fennell, Timothy R; Mortensen, Ninell P; Black, Sherry R et al. (2017) Disposition of intravenously or orally administered silver nanoparticles in pregnant rats and the effect on the biochemical profile in urine. J Appl Toxicol 37:530-544
Mercier, Kelly; McRitchie, Susan; Pathmasiri, Wimal et al. (2017) Preterm neonatal urinary renal developmental and acute kidney injury metabolomic profiling: an exploratory study. Pediatr Nephrol 32:151-161
Laine, Jessica E; Bailey, Kathryn A; Olshan, Andrew F et al. (2017) Neonatal Metabolomic Profiles Related to Prenatal Arsenic Exposure. Environ Sci Technol 51:625-633
Ewald, D Rose; Sumner, Susan C J (2016) Blood type biochemistry and human disease. Wiley Interdiscip Rev Syst Biol Med 8:517-535
Loeser, R F; Pathmasiri, W; Sumner, S J et al. (2016) Association of urinary metabolites with radiographic progression of knee osteoarthritis in overweight and obese adults: an exploratory study. Osteoarthritis Cartilage 24:1479-86
Gelaye, Bizu; Sumner, Susan J; McRitchie, Susan et al. (2016) Maternal Early Pregnancy Serum Metabolomics Profile and Abnormal Vaginal Bleeding as Predictors of Placental Abruption: A Prospective Study. PLoS One 11:e0156755
Wang, Han; Liang, Shuang; Wang, Maoqing et al. (2016) Potential serum biomarkers from a metabolomics study of autism. J Psychiatry Neurosci 41:27-37

Showing the most recent 10 out of 28 publications