The CNPRC has maintained a pedigreed colony of Indian origin rhesus macaques for over 15 years. This Indian origin rhesus population has an extensive pedigree and is self-sustaining with no additional animal recruitment necessary. This application proposes to expand both the number and quality of Indian origin SPF rhesus provided to AIDS funded investigators. This expansion and improvement in animal numbers and quality will be achieved in the following way. 1. Expand production of SPF Level 2 animals by using smaller corn-cribs for smaller, more flexible breeding groups. This will provide greater control over animal production to meet investigator needs. 2. Transfer the current colony database on the CNPRC Vitals computer program to a new LabKey platform that includes electronic medical records as well as a user friendly search function. 3. Develop improved and expanded screening and confirmatory tests for detection of SPF agents. 4. Transition the colony pedigree analysis from the current microsatellite platform to the SNP panel being developed by the Genetics and Genomics Working Group (GGWC) Consortium of the CNPRC. 5. Expand our characterization of the microbiome and immune phenotype of both levels of SPF animals and provide a unique biorepository of fecal and lymphocyte samples which will facilitate future research projects investigating the interaction of HIV with the host microbiome and immune system. The inclusion of the Biorepository Core is in response to research that suggests that knowledge of an individual animal's microbiome may be as important as understanding its MHC haplotype in studying both vaccine response and therapeutic intervention in AIDS related studies.

Public Health Relevance

The CNPRC has a long history in the production of SPF rhesus macaques. The goal of this application is to continue and expand on the improvement and characterization of the model through a program of viral screening, genetic testing, characterization of the enteric microbiome, and most importantly, providing a program of outstanding animal care and management.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
Project #
5U42OD010990-19
Application #
9675352
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Adkins, Ronald
Project Start
2000-09-30
Project End
2020-01-31
Budget Start
2019-02-01
Budget End
2020-01-31
Support Year
19
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of California Davis
Department
Veterinary Sciences
Type
Primate Centers
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Yee, JoAnn L; Grant, Richard; Van Rompay, Koen K et al. (2017) Emerging diagnostic challenges and characteristics of simian betaretrovirus infections in captive macaque colonies. J Med Primatol 46:149-153
Chang, W L William; Gonzalez, Denise F; Kieu, Hung T et al. (2017) Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques. PLoS One 12:e0170154
Yee, JoAnn L; Vanderford, Thomas H; Didier, Elizabeth S et al. (2016) Specific pathogen free macaque colonies: a review of principles and recent advances for viral testing and colony management. J Med Primatol 45:55-78
Oxford, Kristie L; Dela Pena-Ponce, Myra Grace A; Jensen, Kara et al. (2015) The interplay between immune maturation, age, chronic viral infection and environment. Immun Ageing 12:3
Yee, JoAnn L; Montiel, Nestor A; Ardeshir, Amir et al. (2013) Constitutive release of IFN? and IL2 from peripheral blood mononuclear cells of rhesus macaques (Macaca mulatta) infected with simian T-lymphotropic virus type 1. Comp Med 63:508-14