This proposal from the University of California, Davis (UCD) and its subcontractor at CHORI seeks to establish and operate the Knock-Out Mouse Program (KOMP) Repository for the purpose of ensuring the preservation, protection, availability, and accessibility of the KOMP resources for use by the scientific research community. The repository will be built upon the closely linked infrastructure, technical support, and faculty expertise within the UCD Mouse Biology Program, Center for Comparative Medicine, the Schools of Medicine and Veterinary Medicine, and the CHORI BACPAC Resource. UCD shall serve as the lead institution and assume administrative oversight, management, and reporting authority and responsibility for all repository activities. UCD shall archive, maintain, QC, and distribute ES cell clones, live mouse lines, and frozen embryos and sperm, while CHORI shall archive, maintain, QC, and distribute all vectors. Our goals will be to 1) to verify the viability, identity, and utility of the collection;2) make finding and ordering all KOMP products and value-added services simple and easy on-line;and 3) distribute KOMP products promptly and for reasonable cost. To achieve these goals, we shall 1) expand 100% of all gene-targeted ES cell lines to 12-15 vials each;2) genotype, karyotype, and pathogen-screen ES cell clones in each shipment;3) reanimate 425 clones to live mice and test for germline transmission of the mutant allele;4) convert 925 mouse lines (~11% of the collection) to a cryopreserved embryo and sperm archive, and 5) confirm the genotype and SPF-status of each clone when ordered. Requesting investigators will have full access to all UCD resources and services, including reanimation of ES cells into live mice, etc. We shall coordinate our relational databases, internally track KOMP products, interface with the Data Coordinating Center, maintain an easily-navigable and informative public website (, and provide an online searchable catalog and ordering system for all KOMP products, services, and related information. We shall provide attentive customer and technical service to respond to telephone (1-888-KOMP-MICE) and email ( requests for information. Products and services will be offered to requesting investigators for a reasonable fee, enabling us to cost recover for distribution and to develop a self supporting repository operation within 4 years.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
Project #
Application #
Study Section
Special Emphasis Panel (ZHG1-HGR-N (M2))
Program Officer
O'Neill, Raymond R
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Davis
Internal Medicine/Medicine
Schools of Medicine
United States
Zip Code
Feng, Chun-Wei Allen; Spiller, Cassy; Merriner, Donna J et al. (2017) SOX30 is required for male fertility in mice. Sci Rep 7:17619
Kory, Nora; Grond, Susanne; Kamat, Siddhesh S et al. (2017) Mice lacking lipid droplet-associated hydrolase, a gene linked to human prostate cancer, have normal cholesterol ester metabolism. J Lipid Res 58:226-235
Fidler, Trevor P; Middleton, Elizabeth A; Rowley, Jesse W et al. (2017) Glucose Transporter 3 Potentiates Degranulation and Is Required for Platelet Activation. Arterioscler Thromb Vasc Biol 37:1628-1639
Vaeth, Martin; Yang, Jun; Yamashita, Megumi et al. (2017) ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Nat Commun 8:14714
Núnez-Ollé, Marc; Jung, Carole; Terré, Berta et al. (2017) Constitutive Cyclin O deficiency results in penetrant hydrocephalus, impaired growth and infertility. Oncotarget 8:99261-99273
Calabrese, Gina M; Mesner, Larry D; Stains, Joseph P et al. (2017) Integrating GWAS and Co-expression Network Data Identifies Bone Mineral Density Genes SPTBN1 and MARK3 and an Osteoblast Functional Module. Cell Syst 4:46-59.e4
Tran, Melanie; Lee, Sang-Min; Shin, Dong-Ju et al. (2017) Loss of miR-141/200c ameliorates hepatic steatosis and inflammation by reprogramming multiple signaling pathways in NASH. JCI Insight 2:
Lange, Lisette; Marks, Matthias; Liu, Jinhua et al. (2017) Patterning and gastrulation defects caused by the tw18 lethal are due to loss of Ppp2r1a. Biol Open 6:752-764
Fidler, Trevor P; Campbell, Robert A; Funari, Trevor et al. (2017) Deletion of GLUT1 and GLUT3 Reveals Multiple Roles for Glucose Metabolism in Platelet and Megakaryocyte Function. Cell Rep 20:881-894
Vasam, Goutham; Joshi, Shrinidh; Thatcher, Sean E et al. (2017) Reversal of Bone Marrow Mobilopathy and Enhanced Vascular Repair by Angiotensin-(1-7) in Diabetes. Diabetes 66:505-518

Showing the most recent 10 out of 50 publications