The primary goal of the research is to identify the FT and BP genes responsible for several intrinsic antibiotic resistance, virulence and stress tolerance traits. The results should provide mechanistic insights into the physiological basis of each and may identify potential antibacterial drug targets. An additional set of experiments will help define BP species diversity in these and other phenotypes, and may identify diagnostic tests to differentiate natural isolates. As part of the project, a new-generation sequencing-based technology for assessing the makeup of transposon mutant pools of will be developed.

Public Health Relevance

1. Functions required for intrinsic antibiotic resistance and stress resistance are potential drug targets for combination antibacterial therapy. We anticipate that conserved homeostatic functions will be represented and may represent broad host range targets. 2. The analysis of B. pseudomallei phenotypic species variation may identify diagnostic growth tests for discriminating rapidly between natural (non-laboratory) isolates. 3. The F. novicida mutant-phenotype database to be constructed should eventually serve as a reference for identifying the mechanisms of action of new antibacterial compounds (

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Specialized Center--Cooperative Agreements (U54)
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Special Emphasis Panel (ZAI1-DDS-M)
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University of Washington
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West, T Eoin; Myers, Nicolle D; Chantratita, Narisara et al. (2014) NLRC4 and TLR5 each contribute to host defense in respiratory melioidosis. PLoS Negl Trop Dis 8:e3178
Hagar, Jon A; Miao, Edward A (2014) Detection of cytosolic bacteria by inflammatory caspases. Curr Opin Microbiol 17:61-6
Majerczyk, Charlotte D; Brittnacher, Mitchell J; Jacobs, Michael A et al. (2014) Cross-species comparison of the Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei quorum-sensing regulons. J Bacteriol 196:3862-71
Loomis, Wendy P; Johnson, Matthew L; Brasfield, Alicia et al. (2014) Temporal and anatomical host resistance to chronic Salmonella infection is quantitatively dictated by Nramp1 and influenced by host genetic background. PLoS One 9:e111763
Martínez, Luary C; Vadyvaloo, Viveka (2014) Mechanisms of post-transcriptional gene regulation in bacterial biofilms. Front Cell Infect Microbiol 4:38
Myers, Nicolle D; Chantratita, Narisara; Berrington, William R et al. (2014) The role of NOD2 in murine and human melioidosis. J Immunol 192:300-7
Correia, Bruno E; Bates, John T; Loomis, Rebecca J et al. (2014) Proof of principle for epitope-focused vaccine design. Nature 507:201-6
Majerczyk, Charlotte; Brittnacher, Mitchell; Jacobs, Michael et al. (2014) Global analysis of the Burkholderia thailandensis quorum sensing-controlled regulon. J Bacteriol 196:1412-24
Pruneda, Jonathan N; Smith, F Donelson; Daurie, Angela et al. (2014) E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. EMBO J 33:437-49
Sureka, Kamakshi; Choi, Philip H; Precit, Mimi et al. (2014) The cyclic dinucleotide c-di-AMP is an allosteric regulator of metabolic enzyme function. Cell 158:1389-401

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