The main goal of the Core is to generate near-saturation sequence-defined transposon mutant libraries of Yersinia pestis and Burkholderia pseudomallei. The libraries should include mutants with insertions in nearly all genes nonessential for growth on laboratory medium. Mutants from the high coverage libraries (&5 unique insertions/gene) produced initially will be used to create smaller libraries providing relatively complete, redundant (two insertions/gene) coverage of the nonessential genome. The transposons used for mutagenesis will generate lacZ gene fusions, and the libraries will thus serve as sources of both insertion mutations and reporter fusions. The Core will also work to develop methods to transfer transposon mutations from a previously completed Francisella novicida library to Francisella holarctica FSC200 to facilitate genetic studies of a highly virulent Francisella strain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI057141-10
Application #
8447097
Study Section
Special Emphasis Panel (ZAI1-DDS-M)
Project Start
Project End
2015-02-28
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
10
Fiscal Year
2013
Total Cost
$165,373
Indirect Cost
$174,317
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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