The overall objective of this core proposal is to establish a safe, secure and comprehensive Biosafety Level - 3 (BSL3) host-pathogen interaction core to support and enhance the activities of the SERCEB/RCE investigators. The core will provide state-of-the-art flow cytometry and cell sorting, multi-modal live animal imaging, and multiplex biomarker analysis for SERCEB or other RCE investigators running studies in the Regional Biocontainment Laboratory at Duke or for member investigators that send specimens to the core for analysis. To accomplish this goal we have available four base technologies in BSL3 containment: a BDFACS Aria cell sorter, an IVIS-Xenogen Spectrum imager, a BibRad luminex bead array reader and an Abbott Cell Dyn 3700 veterinary hematology analyzer. In support of these base technologies we have offline-computer workstations outside containment, dedicated BSL2 and BSL3 laboratory space for sample preparation, highly trained technical staff in BSL3 and ABSL3 procedures and a PI and Co-investigator with an established track record in immune monitoring research and core facility management. These core technologies are contained at BSL3 in the NIAID-RBL at Duke and run as a core in collaboration with the proposed SERCEB Aerobiology/Animal Models core. This will allow comprehensive host-pathogen analysis at BSL3 under appropriate Standard Operating Procedures for SERCEB/RCE investigators. The use of flow cytometry and in vivo imaging techniques will allow us to monitor both host response and the course of infection, replication, persistence, and dissemination of priority pathogens. Blood and tissue biomarker analysis will provided further valuable insight into host-pathogen interactions which will only enhance the challenge and mechanistic studies proposed by projects in the SERCEB consortium. Areas of active biomedical investigation to be supported by this host-pathogen interation core include fundamental pathogenicity of Priority and Select Agent pathogens, innate and adaptive immunity and host'defense, and development of drugs, diagnostics and vaccines for identified bio-threat pathogens.

Public Health Relevance

This core will establish safe, secure and comprehensive BSL3 host-pathogen interaction monitoring to support the research activities of the SERCEB/RCE investigators. Host-pathogen interaction monitoring will add value to studies proposed in SERCEB, and will allow RCE investigators to better and more rapidly develop the next generation of therapeutics, diagnostics and vaccines against bio-threat pathogens.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI057157-09
Application #
8234182
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2011-03-01
Project End
2014-02-28
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
9
Fiscal Year
2011
Total Cost
$243,890
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Smartt, Chelsea T; Shin, Dongyoung; Alto, Barry W (2017) Dengue serotype-specific immune response in Aedes aegypti and Aedes albopictus. Mem Inst Oswaldo Cruz 112:829-837
Purcell, Erin B; McKee, Robert W; Courson, David S et al. (2017) A Nutrient-Regulated Cyclic Diguanylate Phosphodiesterase Controls Clostridium difficile Biofilm and Toxin Production during Stationary Phase. Infect Immun 85:
Ponnuswamy, Padmapriya; Joffre, Jeremie; Herbin, Olivier et al. (2017) Angiotensin II synergizes with BAFF to promote atheroprotective regulatory B cells. Sci Rep 7:4111
Silva, Laurie A; Dermody, Terence S (2017) Chikungunya virus: epidemiology, replication, disease mechanisms, and prospective intervention strategies. J Clin Invest 127:737-749
Elong Ngono, Annie; Chen, Hui-Wen; Tang, William W et al. (2016) Protective Role of Cross-Reactive CD8 T Cells Against Dengue Virus Infection. EBioMedicine 13:284-293
Bowles, R D; Karikari, I O; VanDerwerken, D N et al. (2016) In vivo luminescent imaging of NF-?B activity and NF-?B-related serum cytokine levels predict pain sensitivities in a rodent model of peripheral neuropathy. Eur J Pain 20:365-76
Rowse, Michael; Qiu, Shihong; Tsao, Jun et al. (2016) Reduction of Influenza Virus Envelope's Fusogenicity by Viral Fusion Inhibitors. ACS Infect Dis 2:47-53
Ashbrook, Alison W; Lentscher, Anthony J; Zamora, Paula F et al. (2016) Antagonism of the Sodium-Potassium ATPase Impairs Chikungunya Virus Infection. MBio 7:
Bates, John T; Pickens, Jennifer A; Schuster, Jennifer E et al. (2016) Immunogenicity and efficacy of alphavirus-derived replicon vaccines for respiratory syncytial virus and human metapneumovirus in nonhuman primates. Vaccine 34:950-6
Alayli, Farah; Scholle, Frank (2016) Dengue virus NS1 enhances viral replication and pro-inflammatory cytokine production in human dendritic cells. Virology 496:227-236

Showing the most recent 10 out of 387 publications