The SERCEB Mouse Monoclonal Antibody Core is housed within the Epitope Recogniton and Immunoreagent Core (ERIC) located at the University of Alabama at Birmingham. The core is responsible for the development and large-scale production of mouse Mabs for use by SERCEB members in studies relating to biodefense and emerging infectious diseases. Services provided by the facility include: procurement and housing of pathogen-free rodents, design and implementation of immunization strategies, sera testing, performance effusions and screening assays, specificity testing, cryopreservation of hybridoma lines, subcloning by limiting dilution, isotyping and large-scale production and purification of Mabs. To be of further service to SERCEB investigators, the core is extending its service to include biotyinylation and enzyme conjugation of Mabs. The Mouse Monoclonal Antibody Core has extensive experience in working with offsite investigators. The core has developed flexible protocols that provide investigators with extended periods of time for screening hybridoma supernatants off campus or in BSL3 facilites if necessary.

Public Health Relevance

The monoclonal antibodies produced in this core facility, will play a crucial role in the detection and characterization of infectious agents that are responsible for human disease. Furthermore, some of the antibodies produced in the core, may provide treatment strategies for infectious agents.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI057157-11
Application #
8437254
Study Section
Special Emphasis Panel (ZAI1-DDS-M)
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
11
Fiscal Year
2013
Total Cost
$154,252
Indirect Cost
$25,085
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Ashbrook, Alison W; Lentscher, Anthony J; Zamora, Paula F et al. (2016) Antagonism of the Sodium-Potassium ATPase Impairs Chikungunya Virus Infection. MBio 7:
Bowles, R D; Karikari, I O; VanDerwerken, D N et al. (2016) In vivo luminescent imaging of NF-κB activity and NF-κB-related serum cytokine levels predict pain sensitivities in a rodent model of peripheral neuropathy. Eur J Pain 20:365-76
Alayli, Farah; Scholle, Frank (2016) Dengue virus NS1 enhances viral replication and pro-inflammatory cytokine production in human dendritic cells. Virology 496:227-36
Bates, John T; Pickens, Jennifer A; Schuster, Jennifer E et al. (2016) Immunogenicity and efficacy of alphavirus-derived replicon vaccines for respiratory syncytial virus and human metapneumovirus in nonhuman primates. Vaccine 34:950-6
Fibriansah, Guntur; Tan, Joanne L; Smith, Scott A et al. (2015) A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins. Nat Commun 6:6341
de St Maurice, Annabelle; Grijalva, Carlos G; Fonnesbeck, Christopher et al. (2015) Racial and Regional Differences in Rates of Invasive Pneumococcal Disease. Pediatrics 136:e1186-94
Roberts, Lydia M; Ledvina, Hannah E; Tuladhar, Shraddha et al. (2015) Depletion of alveolar macrophages in CD11c diphtheria toxin receptor mice produces an inflammatory response. Immun Inflamm Dis 3:71-81
Smith, Scott A; Silva, Laurie A; Fox, Julie M et al. (2015) Isolation and Characterization of Broad and Ultrapotent Human Monoclonal Antibodies with Therapeutic Activity against Chikungunya Virus. Cell Host Microbe 18:86-95
Pulendran, Bali (2015) The varieties of immunological experience: of pathogens, stress, and dendritic cells. Annu Rev Immunol 33:563-606
Price, Aryn A; Sampson, Timothy R; Ratner, Hannah K et al. (2015) Cas9-mediated targeting of viral RNA in eukaryotic cells. Proc Natl Acad Sci U S A 112:6164-9

Showing the most recent 10 out of 375 publications