Regional Center of Excellence (RCE) for Biodefense and Emerging Infectious Disease Research program, the Northeast Biodefense Center (NBC) constitutes a biomedical research program with cores supporting its multifaceted research and development activities. As part of this program, the NBC Proteomics Core will operate within the W.M. Keck Foundation Biotechnology Resource Laboratory at Yale University and will continue to provide a very broad range of bioanalytical services to support basic science, preclinical and clinical research programs in the NBC. In addition, in periods of national biodefense or emerging infectious disease emergency, the NBC Proteomics Core will be prepared and devote all of its considerable resources to meeting the challenge. The NBC Proteomics Core will offer these services from all of its proteomics sections: (1) Mass Spectrometry and Protein Profiling resources that utilize state of the art mass spectrometry instrumentation (LC-FT-ICR, LC-LTQ-Orbitrap, LC-QTRAP-Triple Quadrupole, MALDI TOF/TOF, LC-QTOF, etc) and protein profiling technologies like multidimensional protein identification technology (MudPIT), differential 2-D (fluorescence) gel electrophoresis (DICE), and Multiplexed Isobaric Tagging Technology for Relative and Absolute Quantitation (iTRAQ?). These technologies would also be used in conjunction with the more recently established phosphoprotein profiling methodologies and other protein post translational modification characterization tools. Additionally, this resource will also make available its recently developed clinical proteomics tools for absolute quantitation determination of biomarkers or known biological agents in clinically relevant samples to all NBC investigators. (2) Amino Acid Analysis based on ion-exchange chromatography with post-column ninhydrin detection. (3) Protein Sequencing using Edman (N-terminal) sequencers. (4) A Biophysics Section which offers extensive services including HPLC Size Exclusion Chromatography/laser light scattering determination of native protein molecular weights, (5) Large Scale Peptide Synthesis based on tBOC Chemistry. (6) Small Scale Peptide Synthesis based on Fmoc chemistry with custom peptide modifications. (7) Biostatistics Section offering extensive analysis of proteomic data and improved quantitation algorithms to determine disease biomarkers from mass spectrometric data. With its state-of-the-art instrumentation, and very diverse set of proteomic services, the NBC Proteomics Core staff will consult with members of the NBC regarding the optimum technology to best support the proposed research, experimental design, and interpretation of data produced either by the Proteomics Core or other laboratories to meet the specialized needs of NBC investigators - who will be given the same turn-around priority as Yale investigators

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Specialized Center--Cooperative Agreements (U54)
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Special Emphasis Panel (ZAI1-DDS-M)
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Columbia University (N.Y.)
New York
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Johnson, Joshua C; Martinez, Osvaldo; Honko, Anna N et al. (2014) Pyridinyl imidazole inhibitors of p38 MAP kinase impair viral entry and reduce cytokine induction by Zaire ebolavirus in human dendritic cells. Antiviral Res 107:102-9
Gough, Daniel J; MariƩ, Isabelle J; Lobry, Camille et al. (2014) STAT3 supports experimental K-RasG12D-induced murine myeloproliferative neoplasms dependent on serine phosphorylation. Blood 124:2252-61
Kazakov, Teymur; Kuznedelov, Konstantin; Semenova, Ekaterina et al. (2014) The RimL transacetylase provides resistance to translation inhibitor microcin C. J Bacteriol 196:3377-85
Rajsbaum, Ricardo; Garcia-Sastre, Adolfo; Versteeg, Gijs A (2014) TRIMmunity: the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity. J Mol Biol 426:1265-84
Lo, Michael K; Bird, Brian H; Chattopadhyay, Anasuya et al. (2014) Single-dose replication-defective VSV-based Nipah virus vaccines provide protection from lethal challenge in Syrian hamsters. Antiviral Res 101:26-9
Mackow, Erich R; Dalrymple, Nadine A; Cimica, Velasco et al. (2014) Hantavirus interferon regulation and virulence determinants. Virus Res 187:65-71
Varshney, Avanish K; Wang, Xiaobo; Aguilar, Jorge L et al. (2014) Isotype switching increases efficacy of antibody protection against staphylococcal enterotoxin B-induced lethal shock and Staphylococcus aureus sepsis in mice. MBio 5:e01007-14
Chung, Lawton K; Philip, Naomi H; Schmidt, Valentina A et al. (2014) IQGAP1 is important for activation of caspase-1 in macrophages and is targeted by Yersinia pestis type III effector YopM. MBio 5:e01402-14
DiLillo, David J; Tan, Gene S; Palese, Peter et al. (2014) Broadly neutralizing hemagglutinin stalk-specific antibodies require Fc?R interactions for protection against influenza virus in vivo. Nat Med 20:143-51
Szaba, Frank M; Kummer, Lawrence W; Duso, Debra K et al. (2014) TNF? and IFN? but not perforin are critical for CD8 T cell-mediated protection against pulmonary Yersinia pestis infection. PLoS Pathog 10:e1004142

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