This core is to provide the researchers in the RMRCE with a novel way of testing the results and concepts derived from studies in rodents in human cells before attempting clinical studies in human volunteers or patients. It is our belief that human primary cells are significantly different from human tumor cell lines or primary rodent cells. We believe that these cells, which will be provided by the core, will be very useful to the RMRCE investigators to verify their findings in differentiated primary human lung cells. We isolate lung cells from de-identified human lung donors and will provide alveolar type II cells, alveolar type l-like cells, and alveolar macrophages to RMRCE investigators. Our laboratory has used these cells for infection with SARSCoV and influenza. We will isolate and characterize the cells and train investigators in culturing the epithelial cells. The cells can be frozen down and shipped to the investigators. We have successfully transported these cells to Fort Collins and shipped them to Hong Kong. The alveolar macrophages require no special handing and can be used easily by the investigators. The alveolar epithelial cells require special culture conditions, and investigators will have to spend some time in our lab to learn to prepare the matrices on which the cells are grown. Since one of the major objectives of the RMRCE is to make their discoveries applicable to human subjects and patients, they need access to human cells and tissue. This core will allow access to human lung cells and tissue. This core fits within the RMRCE Strategic Plan by interacting directly with projects in all three of the Integrated Research Focus groups: the Immunomodulation, Adjuvants and Vaccines IRF [RP 1.2 (Jutila), RP 1.3 (Pascual), RP 1.5 (Dow)];the Bacterial Therapeutics IRF [RP 2.7 (Lenz)];and the Viral Therapeutics IRF [RP 3.6 (Li)].
Studies in rodents and rodent cells need to be confirmed in human cells before they can be transferred to the clinic. This core will provide access to human cells and tissues. In addition the investigators of this core have extensive experience in working with and characterizing alveolar epithelial cells and macrophages.
|Gibson, Christopher C; Zhu, Weiquan; Davis, Chadwick T et al. (2015) Strategy for identifying repurposed drugs for the treatment of cerebral cavernous malformation. Circulation 131:289-99|
|Wang, Hong; Siddharthan, Venkatraman; Hall, Jeffery O et al. (2014) Autonomic deficit not the cause of death in West Nile virus neurological disease. Clin Auton Res 24:15-23|
|Scharton, Dionna; Bailey, Kevin W; Vest, Zachary et al. (2014) Favipiravir (T-705) protects against peracute Rift Valley fever virus infection and reduces delayed-onset neurologic disease observed with ribavirin treatment. Antiviral Res 104:84-92|
|Shives, Katherine D; Beatman, Erica L; Chamanian, Mastooreh et al. (2014) West nile virus-induced activation of mammalian target of rapamycin complex 1 supports viral growth and viral protein expression. J Virol 88:9458-71|
|Calvert, Amanda E; Dixon, Kandice L; Delorey, Mark J et al. (2014) Development of a small animal peripheral challenge model of Japanese encephalitis virus using interferon deficient AG129 mice and the SA14-14-2 vaccine virus strain. Vaccine 32:258-64|
|Richert, Laura E; Rynda-Apple, Agnieszka; Harmsen, Ann L et al. (2014) CD11cýýý cells primed with unrelated antigens facilitate an accelerated immune response to influenza virus in mice. Eur J Immunol 44:397-408|
|Soffler, Carl; Bosco-Lauth, Angela M; Aboellail, Tawfik A et al. (2014) Pathogenesis of percutaneous infection of goats with Burkholderia pseudomallei: clinical, pathologic, and immunological responses in chronic melioidosis. Int J Exp Pathol 95:101-19|
|Porta, Jason; Jose, Joyce; Roehrig, John T et al. (2014) Locking and blocking the viral landscape of an alphavirus with neutralizing antibodies. J Virol 88:9616-23|
|Jones-Carson, Jessica; Zweifel, Adrienne E; Tapscott, Timothy et al. (2014) Nitric oxide from IFN?-primed macrophages modulates the antimicrobial activity of ?-lactams against the intracellular pathogens Burkholderia pseudomallei and Nontyphoidal Salmonella. PLoS Negl Trop Dis 8:e3079|
|Phillips, Aaron T; Schountz, Tony; Toth, Ann M et al. (2014) Liposome-antigen-nucleic acid complexes protect mice from lethal challenge with western and eastern equine encephalitis viruses. J Virol 88:1771-80|
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