The majority of NIAID Category A, B, and C pathogens, and nearly all emerging infectious diseases that threaten humans, are zoonotic agents. Thus, animals serve as sources, reservoirs, carriers, and vectors of a wide variety of pathogens. Importantly, research on agents of relevance to biodefense and emerging infectious diseases must use animal models for hypothesis-driven investigation of pathogenesis and immunity, as well as testing and validating vaccine, therapeutic, and diagnostic concepts. DC Davis hosts highly interactive Schools of Medicine and Veterinary Medicine. For the PSW-RCE, the Animal Resources and Laboratory Services Core offers a broad array of animal modeling expertise, technical support, and instrumentation to the scientific community that is involved in responding to national research initiatives in biodefense pathogens and emerging infectious diseases. An important emphasis is on the development of multiplex microbead immunoassays for infectious disease detection through collaborations with other PSW-RCE Projects. A highly interactive collaboration between this Animal Core and the Immunoassay Development Services Core aims to produce a deliverable multiplex diagnostic system for arbovirus serosurveillance. Additionally, the Animal Core coordinates access of RCE investigators to a broad array of existing animal-related resources at DC Davis, including the Center for Comparative Medicine, the Mouse Biology Program, the California National Primate Research Center, the Center for Vector Borne Diseases, and others. The Core provides access for RCE investigators to expertise in comparative and experimental pathology through an innovative telepathology system, with capabilities to link other RCE sites: http://pswrce.compmed.ucdavis.edu/ The Core's mission is to stimulate and facilitate collaborative research interactions among RCE investigators and scientists needing expertise in animal research. These interactions aim to leverage RCE support into independent research programs that foster the NIH research mission in biodefense and emerging infectious disease research. For the renewal grant application, the Core has three major objectives: (1) development of Core infrastructure (expertise, access, prioritization, website, recharge, biosafety training, emergency response network), (2) development of multiplex immunoassay capabilities (serosurveillance of arboviruses via collaboration with the Immunoassay Development Services Core, and (3) support for new collaborative research opportunities in animal models (e.g., West Nile virus, Chikungunga virus, Francisella tularensis) needing animal resources and laboratory

Public Health Relevance

This Core supports animal research on agents of relevance to biodefense and emerging infectious diseases for hypothesis-driven investigation of pathogenesis and immunity, as well as for testing and validating vaccine, therapeutic, and diagnostic concepts. The multiplex microbead immunoassay system developed in this Core will be implemented for surveillance of arbovirus infection in humans;this same system will be adapted for detection of other viral as well as bacterial pathogens studied by PSW-RCE investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI065359-08
Application #
8378825
Study Section
Special Emphasis Panel (ZAI1-DDS-M)
Project Start
Project End
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
8
Fiscal Year
2012
Total Cost
Indirect Cost
Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Waggoner, Jesse J; Gresh, Lionel; Mohamed-Hadley, Alisha et al. (2016) Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses. Emerg Infect Dis 22:1295-7
Ziegler, Christopher M; Eisenhauer, Philip; Bruce, Emily A et al. (2016) The Lymphocytic Choriomeningitis Virus Matrix Protein PPXY Late Domain Drives the Production of Defective Interfering Particles. PLoS Pathog 12:e1005501
Barbour, Alan G (2016) Infection resistance and tolerance in Peromyscus spp., natural reservoirs of microbes that are virulent for humans. Semin Cell Dev Biol :
Park, Arnold; Yun, Tatyana; Hill, Terence E et al. (2016) Optimized P2A for reporter gene insertion into Nipah virus results in efficient ribosomal skipping and wild-type lethality. J Gen Virol 97:839-43
Levin, Mattias; King, Jasmine J; Glanville, Jacob et al. (2016) Persistence and evolution of allergen-specific IgE repertoires during subcutaneous specific immunotherapy. J Allergy Clin Immunol 137:1535-44
Chomel, Bruno B; Molia, Sophie; Kasten, Rickie W et al. (2016) Isolation of Bartonella henselae and Two New Bartonella Subspecies, Bartonellakoehlerae Subspecies boulouisii subsp. nov. and Bartonella koehlerae Subspecies bothieri subsp. nov. from Free-Ranging Californian Mountain Lions and Bobcats. PLoS One 11:e0148299
Kern, Aurelie; Zhou, Chensheng W; Jia, Feng et al. (2016) Live-vaccinia virus encapsulation in pH-sensitive polymer increases safety of a reservoir-targeted Lyme disease vaccine by targeting gastrointestinal release. Vaccine 34:4507-13
Zeltina, Antra; Bowden, Thomas A; Lee, Benhur (2016) Emerging Paramyxoviruses: Receptor Tropism and Zoonotic Potential. PLoS Pathog 12:e1005390
Waggoner, Jesse J; Ballesteros, Gabriela; Gresh, Lionel et al. (2016) Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection. J Clin Virol 78:57-61
Sanman, Laura E; Qian, Yu; Eisele, Nicholas A et al. (2016) Disruption of glycolytic flux is a signal for inflammasome signaling and pyroptotic cell death. Elife 5:e13663

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