We are proposing to develop devices for the quantitative detection of botulinum neurotoxins (BoNT) at attomolar sensitivity with fully automated bench-top instrumentation. We will adopt the existing bead-based BoNT ALISSA technology, developed by the Kalkum lab at City of Hope, into a monolithic microcolumn format to be utilized by common liquid handling robots. We will also develop an automated, highly sensitive, and inexpensive wave-guide-based optical read-out system for integration in the robots. The resulting functional prototype system is expected to automatically process and analyze multiple samples simultaneously and repeatedly (up to 96 per hour). Automated calibration routines and controls for botulinum toxin and non-botulinum toxin proteases will be included. Our assay system will be tested and validated with complex biological matrices such as serum, solid organ and food extracts as well as clinical samples.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI065359-09
Application #
8462547
Study Section
Special Emphasis Panel (ZAI1-DDS-M)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
9
Fiscal Year
2013
Total Cost
$206,743
Indirect Cost
$20,077
Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
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