Autotransporters (ATs) compose a large superfamily of proteins produced by Gram-negative bacteria (47). They consist of an N-terminal signal sequence, a "passenger domain" that mediates the protein's effector function, and a C-terminal "p-domain" involved in translocation of the passenger domain across the outer membrane. "Classical" ATs are monomeric with (3-domains of ~26o aa, while trimeric ATs have (3-domains of -70 aa, three of which form the outer membrane p-barrel structure (47). Classical ATs include the IgA protease ofNeisseria gonorrhoeae, IcsA of Shigellaflexneri, pertactin of Bordetella pertussis, and the serine protease autotransporters of enterpbacteriaceae (SPATEs) (69). Trimeric ATs include YadA of Yersinia species and Hia of Haemophilus influenzae, both of which are involved in adherence (69). Two Partner Secretion (TPS) systems, also widespread among Gram-negative bacteria, are conceptually similar to ATs, except the passenger and p-domains are synthesized as separate proteins known generically as the TpsA and TpsB family members, respectively (104). The TpsA "exoprotein" often is synthesized with a prodomain at either the N- or C-terminus that is removed during translocation, and while some TpsA proteins are released into the extracellular environment, others remain anchored to the cell surface (66,105) ((122,133). Predicting the presence of a pro-domain, whether the mature protein will be released, and, if not, its topology on the cell surface currently is not possible. Functions associated with TpsA proteins include adherence, cytotolysis, heme uptake, and contact-dependent growth inhibition (69). The B. pseudomallei K96243 genome (71) contains 10 putative AT-encoding genes and one TPSencoding locus. One AT, the BPSS1492 or bimA gene product, induces actin polymerization, allowing B. pseudomallei to move within eukaryotic cells'cytoplasm (138). Neither the other AT-encoding OPvFs, nor the genes encoding the putative TPS system, which we named btpA and btpB, have been studied.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI065359-09
Application #
8462549
Study Section
Special Emphasis Panel (ZAI1-DDS-M)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
9
Fiscal Year
2013
Total Cost
$524,057
Indirect Cost
$49,556
Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Torres, Rodrigo; Lan, Benson; Latif, Yama et al. (2014) Structural snapshots along the reaction pathway of Yersinia pestis RipA, a putative butyryl-CoA transferase. Acta Crystallogr D Biol Crystallogr 70:1074-85
Houghton, Raymond L; Reed, Dana E; Hubbard, Mark A et al. (2014) Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis. PLoS Negl Trop Dis 8:e2727
Strotmeier, Jasmin; Mahrhold, Stefan; Krez, Nadja et al. (2014) Identification of the synaptic vesicle glycoprotein 2 receptor binding site in botulinum neurotoxin A. FEBS Lett 588:1087-93
Koellhoffer, Jayne F; Dai, Zhou; Malashkevich, Vladimir N et al. (2014) Structural characterization of the glycoprotein GP2 core domain from the CAS virus, a novel arenavirus-like species. J Mol Biol 426:1452-68
Bennett, Shannon N; Gu, Se Hun; Kang, Hae Ji et al. (2014) Reconstructing the evolutionary origins and phylogeography of hantaviruses. Trends Microbiol 22:473-82
Burtnick, Mary N; Brett, Paul J; DeShazer, David (2014) Proteomic analysis of the Burkholderia pseudomallei type II secretome reveals hydrolytic enzymes, novel proteins, and the deubiquitinase TssM. Infect Immun 82:3214-26
Koskiniemi, Sanna; Garza-Sánchez, Fernando; Sandegren, Linus et al. (2014) Selection of orphan Rhs toxin expression in evolved Salmonella enterica serovar Typhimurium. PLoS Genet 10:e1004255
Sabouri, Amir H; Marcondes, Maria Cecilia Garibaldi; Flynn, Claudia et al. (2014) TLR signaling controls lethal encephalitis in WNV-infected brain. Brain Res 1574:84-95
Vigant, Frederic; Hollmann, Axel; Lee, Jihye et al. (2014) The rigid amphipathic fusion inhibitor dUY11 acts through photosensitization of viruses. J Virol 88:1849-53
Relman, David A (2014) "Inconvenient truths" in the pursuit of scientific knowledge and public health. J Infect Dis 209:170-2

Showing the most recent 10 out of 317 publications