Our initial characterization of Dengue-infection-related immune repertoire has been ofthe B cell repertoires following different modes of infection-based pathology. This work combines a collaborative component (cells and patient information from Eva Harris, Poornima Parameswaran, and their colleagues), experimental manipulations to amplify receptor populations, and computational analysis of amplified sequences from high throughput immunome analysis. The past four months has provided our first detailed look at receptor repertoires in Dengue infection, and to my knowledge the first such analysis anywhere, yielding a number of rather striking observations that suggest both biological hypotheses and experimental followup. Although preliminary, these give a strong indication ofthe value of applying immunome analysis to infectious systems, (i) We observe, as with the original small scale sequencing set carried out in 2010, a strong clonal response in a subset of individuals with pathogenic conditions related to Dengue. (ii)We observe a reproducible appearance of small clones of cells who's appearance coincides with an infectious event but which persist for at least six months after exposure, (iii) We observe different patterns of appearance and maturation in Dengue 2 and Dengue 3 cohorts, suggesting potential mechanisms for the serotype-specific properties of these infectious agents in their effects (short and long-term) on the host. Followup work in this area will provide us the opportunity to determine the generality and significance of these observations and to addresses a series of specific questions related to understanding and management of infection: (i) Will receptor profiling be a valuable adjunct in identifying Dengue-related syndromes and predicting cases that will be severe? (ii) Will receptor profiles of Dengue cases provide specific candidates for immune receptor molecules that mediate unhealthy immune effects during secondary infection? (iii) Will the molecular signatures obtained from this analysis (combined with the extensive analysis in a number of other groups of additional molecular and immunological markers) be a useful clinical tool in guiding

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1-DDS-M)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Irvine
United States
Zip Code
Waggoner, Jesse J; Gresh, Lionel; Mohamed-Hadley, Alisha et al. (2016) Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses. Emerg Infect Dis 22:1295-7
Ziegler, Christopher M; Eisenhauer, Philip; Bruce, Emily A et al. (2016) The Lymphocytic Choriomeningitis Virus Matrix Protein PPXY Late Domain Drives the Production of Defective Interfering Particles. PLoS Pathog 12:e1005501
Barbour, Alan G (2016) Infection resistance and tolerance in Peromyscus spp., natural reservoirs of microbes that are virulent for humans. Semin Cell Dev Biol :
Park, Arnold; Yun, Tatyana; Hill, Terence E et al. (2016) Optimized P2A for reporter gene insertion into Nipah virus results in efficient ribosomal skipping and wild-type lethality. J Gen Virol 97:839-43
Levin, Mattias; King, Jasmine J; Glanville, Jacob et al. (2016) Persistence and evolution of allergen-specific IgE repertoires during subcutaneous specific immunotherapy. J Allergy Clin Immunol 137:1535-44
Chomel, Bruno B; Molia, Sophie; Kasten, Rickie W et al. (2016) Isolation of Bartonella henselae and Two New Bartonella Subspecies, Bartonellakoehlerae Subspecies boulouisii subsp. nov. and Bartonella koehlerae Subspecies bothieri subsp. nov. from Free-Ranging Californian Mountain Lions and Bobcats. PLoS One 11:e0148299
Kern, Aurelie; Zhou, Chensheng W; Jia, Feng et al. (2016) Live-vaccinia virus encapsulation in pH-sensitive polymer increases safety of a reservoir-targeted Lyme disease vaccine by targeting gastrointestinal release. Vaccine 34:4507-13
Zeltina, Antra; Bowden, Thomas A; Lee, Benhur (2016) Emerging Paramyxoviruses: Receptor Tropism and Zoonotic Potential. PLoS Pathog 12:e1005390
Waggoner, Jesse J; Ballesteros, Gabriela; Gresh, Lionel et al. (2016) Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection. J Clin Virol 78:57-61
Sanman, Laura E; Qian, Yu; Eisele, Nicholas A et al. (2016) Disruption of glycolytic flux is a signal for inflammasome signaling and pyroptotic cell death. Elife 5:e13663

Showing the most recent 10 out of 434 publications