The overall goal of this multi-disciplinary 2-year project is to engage an active collaborative partnership between researchers in atmospheric chemistry and infectious diseases for development of rapid diagnostic tools based on the detection and identification of informative trace gases in the exhaled breath and/or blood from individuals with infection. We first carry-out investigations of experimental animal models of infection, first at the BSL2 level of containment and then, as procedures become refined, at the BSL3 level.
The specific aims are the following: (1) Further development of gas signature detection and analysis using animal models of Borrelia and Salmonella. Samples of exhaled breath and blood from the infected animals and control animals will be analyzed using advanced gas chromatographic techniques with one-of-a-kind capability for broad and reproducible detection of volatile organic compounds in the parts per trillion range. We also are assessing the contributions of selected other variables besides infection on trace gases in the exhaled breath and blood. These include age, sex, MHC background, and normal flora. Once there has been characterization with one or more models of bacterial infection of mice, we will extend the analysis to selected viral infections of experimental models at the BSL2 level. (2) Application of gas signature analysis to experimental animal models of pulmonary and systemic infection with Burkholderia pseudomailei in first mice and then hamsters as a second species. Gas signatures will be correlated with pathogen burdens and distributions in the animals, disease severity and outcome, and various biomarkers of host responses. We anticipate that some informative gas signatures will be attributable to host response to infection or to a unique interaction between pathogen and mammalian host.

Public Health Relevance

Recent advances from studies of chemicals in very low concentrations in the atmosphere will be applied to the analysis ofthe exhaled breath and blood from laboratory animals with dififerent types of bacterial and viral Infections. There is the potential that the rapid detection of gases that are characteristic of certain types of infections could revolutionize the field of infectious diseases diagnosis.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1-DDS-M)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Irvine
United States
Zip Code
Torres, Rodrigo; Lan, Benson; Latif, Yama et al. (2014) Structural snapshots along the reaction pathway of Yersinia pestis RipA, a putative butyryl-CoA transferase. Acta Crystallogr D Biol Crystallogr 70:1074-85
Houghton, Raymond L; Reed, Dana E; Hubbard, Mark A et al. (2014) Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis. PLoS Negl Trop Dis 8:e2727
Strotmeier, Jasmin; Mahrhold, Stefan; Krez, Nadja et al. (2014) Identification of the synaptic vesicle glycoprotein 2 receptor binding site in botulinum neurotoxin A. FEBS Lett 588:1087-93
Koellhoffer, Jayne F; Dai, Zhou; Malashkevich, Vladimir N et al. (2014) Structural characterization of the glycoprotein GP2 core domain from the CAS virus, a novel arenavirus-like species. J Mol Biol 426:1452-68
Bennett, Shannon N; Gu, Se Hun; Kang, Hae Ji et al. (2014) Reconstructing the evolutionary origins and phylogeography of hantaviruses. Trends Microbiol 22:473-82
Burtnick, Mary N; Brett, Paul J; DeShazer, David (2014) Proteomic analysis of the Burkholderia pseudomallei type II secretome reveals hydrolytic enzymes, novel proteins, and the deubiquitinase TssM. Infect Immun 82:3214-26
Koskiniemi, Sanna; Garza-Sánchez, Fernando; Sandegren, Linus et al. (2014) Selection of orphan Rhs toxin expression in evolved Salmonella enterica serovar Typhimurium. PLoS Genet 10:e1004255
Sabouri, Amir H; Marcondes, Maria Cecilia Garibaldi; Flynn, Claudia et al. (2014) TLR signaling controls lethal encephalitis in WNV-infected brain. Brain Res 1574:84-95
Vigant, Frederic; Hollmann, Axel; Lee, Jihye et al. (2014) The rigid amphipathic fusion inhibitor dUY11 acts through photosensitization of viruses. J Virol 88:1849-53
Relman, David A (2014) "Inconvenient truths" in the pursuit of scientific knowledge and public health. J Infect Dis 209:170-2

Showing the most recent 10 out of 317 publications