Cancer is one of the most serious diseases in man and the second most fatal disease, after atherosclerosis. Breast cancer is the most common cancer among African American w/omen. Recentiy, interest in herbal extracts for the prevention and treatment of this disease has increased, for such extracts are considered to be safe, economically feasible, and effective. Withania somnifera (WS), commonly known as ashwagandha, has been used for many years in traditional medicine, especially in the treatment of human tumors, arthritis, and stress. This small, woody shrub, which grows about two feet in height, is found in Africa, the Mediterranean, India, and the United States, where it is known as winter cherry. Preliminary data from our laboratory, obtained on a previous pilot project, funded by the MSM/TU/UAB CCC Partnership, show that daily administration of a root extract of WS (given as an oral dose of 300 mg/kg for 21 days) causes 99% growth inhibition of breast cancer cells xenografted in mice. The main goal ofthis proposal is to determine a safe and efficacious dose of WS for use in rats for chemoprevention in methylnitrosourea (MNU)-induced mammary tumors and in mice for therapy of xenografted breast cancer cells. In addition, the effect ofthe WS root extract, alone and in combination with tamoxifen, will be evaluated in a panel of breast cancer cell lines in vitro. The molecular effects of WS extract will be evaluated by using slices of tumors obtained from treated animals and breast cancer cells exposed to the extract.

Public Health Relevance

This study could provide a basis for the clinical use of a safe and efficacious anti-tumor drug. By reducing the doses of standard drugs and the cost of treatment, such a drug could provide effective therapy and also reduce the suffering of breast cancer patients caused by the side effects of currently used anti-cancer medicines and treatments.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA118623-08
Application #
8567089
Study Section
Special Emphasis Panel (ZCA1-SRLB-Y)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
8
Fiscal Year
2013
Total Cost
$270,433
Indirect Cost
$100,768
Name
Tuskegee University
Department
Type
DUNS #
128214178
City
Tuskegee
State
AL
Country
United States
Zip Code
36088
Wang, Honghe; Liu, Wei; Black, ShaNekkia et al. (2016) Kaiso, a transcriptional repressor, promotes cell migration and invasion of prostate cancer cells through regulation of miR-31 expression. Oncotarget 7:5677-89
Sehovic, Ivana; Gwede, Clement K; Meade, Cathy D et al. (2016) A Web-Based Platform for Educating Researchers About Bioethics and Biobanking. J Cancer Educ 31:397-404
Simon, Liz; Song, Keijing; Vande Stouwe, Curtis et al. (2016) Δ9-Tetrahydrocannabinol (Δ9-THC) Promotes Neuroimmune-Modulatory MicroRNA Profile in Striatum of Simian Immunodeficiency Virus (SIV)-Infected Macaques. J Neuroimmune Pharmacol 11:192-213
Jones, Jacqueline; Mukherjee, Angana; Karanam, Balasubramanyam et al. (2016) African Americans with pancreatic ductal adenocarcinoma exhibit gender differences in Kaiso expression. Cancer Lett 380:513-22
Sodeke, Stephen (2016) Bioethics Skill Sets Can Work, But It Would Take Moral Courage to Apply Them and Get Desired Results. Am J Bioeth 16:19-21
Rust, George; Zhang, Shun; Malhotra, Khusdeep et al. (2015) Paths to health equity: Local area variation in progress toward eliminating breast cancer mortality disparities, 1990-2009. Cancer 121:2765-74
Tomita, Saori; Abdalla, Mohamed Osama Ali; Fujiwara, Saori et al. (2015) A cluster of noncoding RNAs activates the ESR1 locus during breast cancer adaptation. Nat Commun 6:6966
Reams, R Renee; Jones-Triche, Jacqueline; Chan, Owen T M et al. (2015) Immunohistological analysis of ABCD3 expression in Caucasian and African American prostate tumors. Biomed Res Int 2015:132981
Wang, Lizhong; Liu, Runhua; Ye, Peiying et al. (2015) Intracellular CD24 disrupts the ARF-NPM interaction and enables mutational and viral oncogene-mediated p53 inactivation. Nat Commun 6:5909
Cooke, Paul S; Simon, Liz; Nanjappa, Manjunatha K et al. (2015) Plasticity of spermatogonial stem cells. Asian J Androl 17:355-9

Showing the most recent 10 out of 110 publications