7.2.3. Core C. Clinical studies and molecular pathology Core Leader: Jacques Van Dam Investigators: James Crawford, Jay Pasricha, Shiro Urayama Clinical studies - testing of new probes The testing of new probes will be undertaken first in small animal models (to determine the focality of the uptake) and then in large animals using endoscopic imaging techniques. Both safety and efficacy will be judged using animal models. As small animals and even large animal models do not perfectly predict biological behavior in humans, we will move to human studies once safety and efficacy in animals have been established. Peptide probes will be administered topically during routine endoscopy (Hsiung P. et al., 2008). Target tissue (polyps, inflammation, control regions of relatively normal mucosa) will be washed with water to remove excess mucus. Approximately 3-6 mL of peptide at a concentration of 100 uM will be topically administered using a standard spray catheter (Olympus washing pipe, Olympus America, Inc.;Center Valley, PA). Excess peptide will be rinsed away and imaging undertaken. For deeper target structures such as neoplastic lesions (esophageal, gastric, rectal cancers) and associated enlarged lymph nodes of uncertain nature (benign, inflammatory, metastatic), peptide probes will require parenteral administration. Again, safety and efficacy will be judged in animal models prior to human study. Due to the circulating time, it is anticipated that peptides will require administration in advanced of the imaging procedure. The optimal time of localization of peptide with the greatest signal- to-noise ratio will be determined in animal models prior to human study. Once approved for human study, patient selection will include those with known gastrointestinal malignancy referred for endosonographic staging procedures and, as controls, those patients with inflammatory conditions (esophagitis, colitis, gastritis) in which enlarged lymph nodes may be inflammatory rather than neoplastic. Many cancer patients will then be referred for surgical resection and lymph node harvest. Excised lymph nodes will be assess for metastatic disease (standard of care) and will serve as """"""""gold standard."""""""" Many patients with benign disease (fulminant colitis, chronic pancreatitis) will also undergo surgical resection and again, harvested lymph nodes will be histopathologically assessed.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZCA1-SRRB-9)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Stanford University
United States
Zip Code
Uddin, Md Jashim; Moore, Chauca E; Crews, Brenda C et al. (2016) Fluorocoxib A enables targeted detection of cyclooxygenase-2 in laser-induced choroidal neovascularization. J Biomed Opt 21:90503
Uddin, Md Jashim; Werfel, Thomas A; Crews, Brenda C et al. (2016) Fluorocoxib A loaded nanoparticles enable targeted visualization of cyclooxygenase-2 in inflammation and cancer. Biomaterials 92:71-80
Sensarn, Steven; Zavaleta, Cristina L; Segal, Ehud et al. (2016) A Clinical Wide-Field Fluorescence Endoscopic Device for Molecular Imaging Demonstrating Cathepsin Protease Activity in Colon Cancer. Mol Imaging Biol 18:820-829
Templeton, Zachary S; Bachmann, Michael H; Alluri, Rajiv V et al. (2015) Methods for culturing human femur tissue explants to study breast cancer cell colonization of the metastatic niche. J Vis Exp :
Uddin, Md Jashim; Crews, Brenda C; Ghebreselasie, Kebreab et al. (2015) Targeted imaging of cancer by fluorocoxib C, a near-infrared cyclooxygenase-2 probe. J Biomed Opt 20:50502
Rogalla, Stephan; Contag, Christopher H (2015) Early Cancer Detection at the Epithelial Surface. Cancer J 21:179-87
Garai, Ellis; Sensarn, Steven; Zavaleta, Cristina L et al. (2015) A real-time clinical endoscopic system for intraluminal, multiplexed imaging of surface-enhanced Raman scattering nanoparticles. PLoS One 10:e0123185
Ra, Hyejun; González-González, Emilio; Uddin, Md Jashim et al. (2015) Detection of non-melanoma skin cancer by in vivo fluorescence imaging with fluorocoxib A. Neoplasia 17:201-7
Contag, Christopher H; Lie, Wen-Rong; Bammer, Marie C et al. (2014) Monitoring dynamic interactions between breast cancer cells and human bone tissue in a co-culture model. Mol Imaging Biol 16:158-66
Ajithkumar, G; Yoo, Benjamin; Goral, Dara E et al. (2013) Multimodal bioimaging using rare earth doped Gd2O2S: Yb/Er phosphor with upconversion luminescence and magnetic resonance properties. J Mater Chem A Mater 1:1561-1572

Showing the most recent 10 out of 32 publications