The proposed Center will make extensive use of a new Microfluidic Facility for the fabrication, assembly, and use of Microhabitat patches towards cancer and evolution experiments. A major goal for the proposed center is to facilitate the transfer of capabilities and knowledge from the physical sciences to biologists and medical researchers to learn more about cancer. Specifically, these capabilities are the """"""""Microhabitat patches"""""""" (MHP) for studying the evolution of cancer cells under stress. These MHP's are microfluidic chips. Thus we are developing a staffed facility for biologists to make and to use microfluidic chips in general and microhabitat patches in particular for this proposed center. The facility consists of two functional pieces - one for making and packaging the microfluidic chips, and another for using the chips to do experiments (in this case biological with a cancer focus). Both of these functional pieces will be set up for remote operation and web interfaces, so that team members at institutions besides Princeton can make use of them. The facility will also be heavily used by the outreach and education/training sections of the center, and we expect by the pilot and transnetwork projects as well.

Public Health Relevance

The proposed Center will make extensive use of a new Microfluidic Facility for the fabrication, assembly, and use of Microhabitat patches towards cancer and evolution experiments. The facility consists of two functional pieces - one for making and packaging the microfluidic chips, and another for using the chips to do experiments (in this case biological with a cancer focus).

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA143803-05
Application #
8535650
Study Section
Special Emphasis Panel (ZCA1-SRLB-9)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
5
Fiscal Year
2013
Total Cost
$116,501
Indirect Cost
Name
Princeton University
Department
Type
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
Jung, Younghun; Decker, Ann M; Wang, Jingcheng et al. (2016) Endogenous GAS6 and Mer receptor signaling regulate prostate cancer stem cells in bone marrow. Oncotarget 7:25698-711
Han, Weijing; Chen, Shaohua; Yuan, Wei et al. (2016) Oriented collagen fibers direct tumor cell intravasation. Proc Natl Acad Sci U S A 113:11208-11213
Yumoto, Kenji; Eber, Matthew R; Wang, Jingcheng et al. (2016) Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep 6:36520
Piotrowski-Daspit, Alexandra S; Tien, Joe; Nelson, Celeste M (2016) Interstitial fluid pressure regulates collective invasion in engineered human breast tumors via Snail, vimentin, and E-cadherin. Integr Biol (Camb) 8:319-31
Gascard, Philippe; Tlsty, Thea D (2016) Carcinoma-associated fibroblasts: orchestrating the composition of malignancy. Genes Dev 30:1002-19
Amend, Sarah R; Roy, Sounak; Brown, Joel S et al. (2016) Ecological paradigms to understand the dynamics of metastasis. Cancer Lett 380:237-42
van der Toom, Emma E; Verdone, James E; Pienta, Kenneth J (2016) Disseminated tumor cells and dormancy in prostate cancer metastasis. Curr Opin Biotechnol 40:9-15
Amend, Sarah R; Valkenburg, Kenneth C; Pienta, Kenneth J (2016) Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation. J Vis Exp :
Lee, Eunsohl; Wang, Jingcheng; Yumoto, Kenji et al. (2016) DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis. Neoplasia 18:553-66
Pan, Deng; Roy, Somdutta; Gascard, Philippe et al. (2016) SOX2, OCT3/4 and NANOG expression and cellular plasticity in rare human somatic cells requires CD73. Cell Signal 28:1923-1932

Showing the most recent 10 out of 84 publications