The purpose ofthis Shared Resource is to provide specialized equipment and techniques for using sequencing methods to analyze gene expression, DNA methylation states, microRNA signatures, and gene translocation/duplication in the genome. This information will be used to better understand novel cancer pathways at the single cell level, including the rapid cell evolution response that allows some cancer cells to sun/ive chemotherapy. This Shared Resource will expand upon current methodology of sequencing a few cells, bringing high throughput sequencing and other services and expertise to each Project. The proposed procedure involves optimization of all steps, from isolation and lysis of, ultimately, a single cell, to whole genome amplification and analysis.

Public Health Relevance

This Shared Resource will expand upon current methodology of sequencing a few cells, bringing high throughput sequencing and other services and expertise to each Project. The proposed procedure involves optimization of all steps, from isolation and lysis of, ultimately, a single cell, to whole genome amplification and analysis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA143803-05
Application #
8535653
Study Section
Special Emphasis Panel (ZCA1-SRLB-9)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
5
Fiscal Year
2013
Total Cost
$120,915
Indirect Cost
Name
Princeton University
Department
Type
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
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