The purpose ofthis Shared Resource is to provide specialized equipment and techniques for using sequencing methods to analyze gene expression, DNA methylation states, microRNA signatures, and gene translocation/duplication in the genome. This information will be used to better understand novel cancer pathways at the single cell level, including the rapid cell evolution response that allows some cancer cells to sun/ive chemotherapy. This Shared Resource will expand upon current methodology of sequencing a few cells, bringing high throughput sequencing and other services and expertise to each Project. The proposed procedure involves optimization of all steps, from isolation and lysis of, ultimately, a single cell, to whole genome amplification and analysis.

Public Health Relevance

This Shared Resource will expand upon current methodology of sequencing a few cells, bringing high throughput sequencing and other services and expertise to each Project. The proposed procedure involves optimization of all steps, from isolation and lysis of, ultimately, a single cell, to whole genome amplification and analysis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA143803-05
Application #
8535653
Study Section
Special Emphasis Panel (ZCA1-SRLB-9)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
5
Fiscal Year
2013
Total Cost
$120,915
Indirect Cost
Name
Princeton University
Department
Type
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
van Vliet, Simon; Hol, Felix J H; Weenink, Tim et al. (2014) The effects of chemical interactions and culture history on the colonization of structured habitats by competing bacterial populations. BMC Microbiol 14:116
Yang, Kimberline R; Mooney, Steven M; Zarif, Jelani C et al. (2014) Niche inheritance: a cooperative pathway to enhance cancer cell fitness through ecosystem engineering. J Cell Biochem 115:1478-85
Wan, Liling; Hu, Guohong; Wei, Yong et al. (2014) Genetic ablation of metadherin inhibits autochthonous prostate cancer progression and metastasis. Cancer Res 74:5336-47
DeFilippis, Rosa Anna; Fordyce, Colleen; Patten, Kelley et al. (2014) Stress signaling from human mammary epithelial cells contributes to phenotypes of mammographic density. Cancer Res 74:5032-44
Lee, Mei-Chong Wendy; Lopez-Diaz, Fernando J; Khan, Shahid Yar et al. (2014) Single-cell analyses of transcriptional heterogeneity during drug tolerance transition in cancer cells by RNA sequencing. Proc Natl Acad Sci U S A 111:E4726-35
Terada, Naoki; Shiraishi, Takumi; Zeng, Yu et al. (2014) Correlation of Sprouty1 and Jagged1 with aggressive prostate cancer cells with different sensitivities to androgen deprivation. J Cell Biochem 115:1505-15
Chen, Duyu; Jiao, Yang; Torquato, Salvatore (2014) A cellular automaton model for tumor dormancy: emergence of a proliferative switch. PLoS One 9:e109934
Roca, Hernan; Pande, Manjusha; Huo, Jeffrey S et al. (2014) A bioinformatics approach reveals novel interactions of the OVOL transcription factors in the regulation of epithelial - mesenchymal cell reprogramming and cancer progression. BMC Syst Biol 8:29
Fuhrmann, Alexander; Li, Julie; Chien, Shu et al. (2014) Cation type specific cell remodeling regulates attachment strength. PLoS One 9:e102424
Khin, Zayar P; Ribeiro, Maria L C; Jacobson, Timothy et al. (2014) A preclinical assay for chemosensitivity in multiple myeloma. Cancer Res 74:56-67

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